Anti-NFIB / NF1B2抗体[NFI5I299] (ab51352)

概述

  • 产品名称Anti-NFIB / NF1B2抗体[NFI5I299]
    参阅全部 NFIB / NF1B2 一抗
  • 描述
    小鼠单克隆抗体[NFI5I299] to NFIB / NF1B2
  • 经测试应用适用于: Dot blot, IP, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment (Human)

  • 阳性对照
    • HeLa cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 micrometer membrane.
  • 克隆单克隆
  • 克隆编号NFI5I299
  • 同种型IgG1
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab51352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot Use at an assay dependent dilution.
IP Use at 20 µg/mg of lysate.
WB 1/100. Detects a band of approximately 49 kDa (predicted molecular weight: 47 kDa).

靶标

  • 功能Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
  • 序列相似性Belongs to the CTF/NF-I family.
    Contains 1 CTF/NF-I DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CCAAT Box Binding Transcription Factor antibody
    • CCAAT-box-binding transcription factor antibody
    • CTF antibody
    • HMGIC/NFIB antibody
    • NF-I/B antibody
    • NF1-B antibody
    • NF1B antibody
    • NF1B2 antibody
    • NFI-B antibody
    • NFI-RED antibody
    • Nfib antibody
    • NFIB_HUMAN antibody
    • NFIB2 antibody
    • NFIB3 antibody
    • Nuclear factor 1 B-type antibody
    • Nuclear factor 1/B antibody
    • Nuclear Factor 1B antibody
    • Nuclear Factor I B antibody
    • Nuclear factor I/B antibody
    • TGGCA Binding Protein antibody
    • TGGCA-binding protein antibody
    • TRANSCRIPTION FACTOR NFIB antibody
    see all

Anti-NFIB / NF1B2 antibody [NFI5I299] 图像

  • ab51252 used at 10µg/500µg RIPA lysate of HeLa cells. Western blot labelled with ab51352 at 1/100 dilution followed by TrueBlot anti Mouse IgG at 1/1000 dilution. Developed using the ECL technique.

Anti-NFIB / NF1B2 antibody [NFI5I299] (ab51352)参考文献

ab51352 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (E17 Mouse brain slices, free floating)
Specification E17 Mouse brain slices, free floating
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization Yes - 0.2% Triton X
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Dr. Randal Moldrich

Verified customer

提交于 Jul 08 2009

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (E17 Mouse brain slices)
Specification E17 Mouse brain slices
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton X
Blocking step Goat serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Dr. Randal Moldrich

Verified customer

提交于 Jul 08 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"