Anti-NFIB / NF1B2抗体(ab11989)


  • 产品名称Anti-NFIB / NF1B2抗体
    参阅全部 NFIB / NF1B2 一抗
  • 描述
    兔多克隆抗体to NFIB / NF1B2
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Hamster
  • 免疫原

    Synthetic peptide:


    , corresponding to amino acids 402-415 of Human NF1B2

  • 阳性对照
    • MG-63 nuclear extract.



Our Abpromise guarantee covers the use of ab11989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). A doublet may be seen in some cell lines. For optimal results, primary antibody incubations should be performed at room temperature (see recommended protocol). The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background.


Anti-NFIB / NF1B2 antibody 图像

  • Predicted band size : 47 kDa

    NF1B2 detection by Western blot analysis. NF1B2 is detected in Rat Liver cells using NF1B2 rabbit polyclonal antibody at a 1/1000 dilution (lane 1). The signal is eliminated by adding the immunizing peptide to the reaction mixture (lane 2).
  • ab11989 staining of NFIB/NF1B2 in mouse cortex E15. PFA-fixed paraffin-embedded sections of mouse cortex E15 were incubated with ab11989 (1/1000) for 18 hours at 4°C. Antigen retrieval was performed by heat induction in sodium citrate buffer. Please see accompanying abreview for additional information.

    See Abreview

Anti-NFIB / NF1B2 antibody (ab11989)参考文献

This product has been referenced in:

See all 2 Publications for this product

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The FOCR for order number PO-14643 is 1111586.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mouse cortex E15)
Specification Mouse cortex E15
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization Yes - 0.025% TX100
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C

Abcam user community

Verified customer

提交于 Jul 23 2008

Thank you for your e-mail. The source of the antibody told us " I am not sure if the 3 isoforms are expressed simultaneously in rat liver nuclear extract and I think that's not the case". We hope this information will clarify the reason for 1 band...

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The source of the antibody ab11989 has informed us " The antibody is raised against the peptide YVPSYDPSSPQTSQ. From our blast results, it seems the antibody should recognize all 3 isoforms". We hope this information will help you, please do not hes...

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