概述

  • 产品名称Anti-NFIA抗体
    参阅全部 NFIA 一抗
  • 描述
    兔多克隆抗体to NFIA
  • 经测试应用适用于: WB, IHC-Fr, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human NFIA.

    (Peptide available as ab45354.)

  • 阳性对照
    • ab41851 gave a positive result in the following Human Whole Cell Lysates: Hela, Jurkat, A431, HEK293, HepG2 and MCF-7. In addition, ab41851 gave a postive result in the following Tissue Lysates: Human Liver, Mouse Liver, Rat Liver (Data not shown), Rat Brain (Data not shown).

性能

应用

Our Abpromise guarantee covers the use of ab41851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-Fr
ICC/IF
  • 应用说明ICC/IF: Use at a concentration of 1 µg/ml.
    IHC-Fr: See Abreview for dilution recommendations
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 56 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
    • 序列相似性Belongs to the CTF/NF-I family.
      Contains 1 CTF/NF-I DNA-binding domain.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 1110047K16Rik antibody
      • 9430022M17Rik antibody
      • CCAAT Box Binding Transcription Factor antibody
      • CCAAT-box-binding transcription factor antibody
      • CTF antibody
      • FLJ39164 antibody
      • NF I/A antibody
      • NF-I/A antibody
      • NF1-A antibody
      • NFI A antibody
      • NFI L antibody
      • NFI-A antibody
      • NFIA antibody
      • NFIA_HUMAN antibody
      • Nuclear factor 1 A type antibody
      • Nuclear factor 1 A-type antibody
      • Nuclear factor 1/A antibody
      • Nuclear factor I/A antibody
      • TGGCA binding protein antibody
      • TGGCA-binding protein antibody
      see all

    Anti-NFIA antibody 图像

    • All lanes : Anti-NFIA antibody (ab41851) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
      Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
      Lane 7 : Human liver tissue lysate - total protein (ab29889)
      Lane 8 : Liver (Mouse) Mouse Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 56 kDa
      Observed band size : 54 kDa (why is the actual band size different from the predicted?)
    • ICC/IF image of ab41851 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab41851, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

    • Ab41851 staining NFIA in motorneurons of rat brain tissue sections by IHC-Fr.  Sections were PFA fixed and incubated with ab41851, diluted 1/100, for 24 hours at 4°C. Chromeo™ 488 conjugated goat polyclonal to rabbit IgG ab60314, diluted 1/2000, was used as the secondary antibody.

      See Abreview

    Anti-NFIA antibody (ab41851)参考文献

    This product has been referenced in:
    • Nagao M  et al. Zbtb20 promotes astrocytogenesis during neocortical development. Nat Commun 7:11102 (2016). WB ; Mouse . Read more (PubMed: 27000654) »
    • Serio A  et al. Astrocyte pathology and the absence of non-cell autonomy in an induced pluripotent stem cell model of TDP-43 proteinopathy. Proc Natl Acad Sci U S A 110:4697-702 (2013). ICC/IF ; Human . Read more (PubMed: 23401527) »

    See all 4 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Rat Tissue sections (brain sections)
    Specification brain sections
    Fixative Paraformaldehyde
    Permeabilization No
    Username

    Dr. Sophie Pezet

    Verified customer

    提交于 Jun 30 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"