重组
RabMAb

Anti-NFAT1抗体[EPR2973] (ab92490)

概述

  • 产品名称
    Anti-NFAT1抗体[EPR2973]
    参阅全部 NFAT1 一抗
  • 描述
    兔单克隆抗体[EPR2973] to NFAT1
  • 经测试应用
    适用于: WBmore details
    不适用于: ICC,IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human NFAT1 (UniProt Q13469).

  • 阳性对照
    • Jurkat cell lysate, Ramos cell lysate
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab92490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明
    WB: 1/1000 - 1/5000. Detects a band of approximately 135 kDa (predicted molecular weight: 100 kDa).

    Is unsuitable for ICC, IHC-P or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF.
    • 组织特异性
      Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas.
    • 序列相似性
      Contains 1 RHD (Rel-like) domain.
    • 结构域
      Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
    • 翻译后修饰
      In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated; which is required for full transcriptional activity.
    • 细胞定位
      Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AI607462 antibody
      • cytoplasmic 2 antibody
      • KIAA0611 antibody
      • NF ATc2 antibody
      • NF ATp antibody
      • NF-ATc2 antibody
      • NF-ATp antibody
      • NFAC2_HUMAN antibody
      • NFAT 1 antibody
      • NFAT pre existing subunit antibody
      • NFAT pre-existing subunit antibody
      • NFAT transcription complex, preexisting component antibody
      • NFAT1 antibody
      • NFAT1-D antibody
      • NFATc2 antibody
      • NFATp antibody
      • Nuclear factor of activated T cells cytoplasmic 2 antibody
      • Nuclear factor of activated T cells cytoplasmic calcineurin dependent 2 antibody
      • Nuclear factor of activated T cells pre-existing component antibody
      • Nuclear factor of activated T cells, preexisting component antibody
      • Nuclear factor of activated T-cells antibody
      • Preexisting nuclear factor of activated T cells 2 antibody
      • T cell transcription factor NFAT 1 antibody
      • T-cell transcription factor NFAT1 antibody
      see all

    图片

    • All lanes : Anti-NFAT1 antibody [EPR2973] (ab92490) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Ramos cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 100 kDa
      Observed band size : 135 kDa (why is the actual band size different from the predicted?)

    实验方案

    文献

    ab92490 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    I am very pleased to hear you would like to accept our offer and test ab92490 in ChIP. This code will give you the VALUE of this product off an order before the expiration date. To redeem this offer, please submit an Abreview for ChIP. The code is inac...

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    Thank you very much for your interest in ab92490.

    To our knowledge, this product nor any of our other anti-NFAT products, have not been tested in ChIP. However by participating in our AbTrial program, you can now use our products in an unt...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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