概述

  • 产品名称Anti-NFAT1抗体
    参阅全部 NFAT1 一抗
  • 描述
    山羊多克隆抗体to NFAT1
  • 特异性This antibody is expected to recognize all three reported isoforms (NP_036472.2; NP_775114.1; NP_001129493.1).
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    QQFRTGLSSPDARYQ

    by a Cysteine residue linker, corresponding to internal sequence amino acids 729 - 743 of Human NFAT1 (NP_036472.2; NP_775114.1; NP_001129493.1)

  • 阳性对照
    • MOLT4 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab95479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 3 - 6 µg/ml.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 100 kDa).

靶标

  • 功能Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF.
  • 组织特异性Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas.
  • 序列相似性Contains 1 RHD (Rel-like) domain.
  • 结构域Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
  • 翻译后修饰In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated; which is required for full transcriptional activity.
  • 细胞定位Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI607462 antibody
    • cytoplasmic 2 antibody
    • KIAA0611 antibody
    • NF ATc2 antibody
    • NF ATp antibody
    • NF-ATc2 antibody
    • NF-ATp antibody
    • NFAC2_HUMAN antibody
    • NFAT 1 antibody
    • NFAT pre existing subunit antibody
    • NFAT pre-existing subunit antibody
    • NFAT transcription complex, preexisting component antibody
    • NFAT1 antibody
    • NFAT1-D antibody
    • NFATc2 antibody
    • NFATp antibody
    • Nuclear factor of activated T cells cytoplasmic 2 antibody
    • Nuclear factor of activated T cells cytoplasmic calcineurin dependent 2 antibody
    • Nuclear factor of activated T cells pre-existing component antibody
    • Nuclear factor of activated T cells, preexisting component antibody
    • Nuclear factor of activated T-cells antibody
    • Preexisting nuclear factor of activated T cells 2 antibody
    • T cell transcription factor NFAT 1 antibody
    • T-cell transcription factor NFAT1 antibody
    see all

Anti-NFAT1 antibody 图像

  • Anti-NFAT1 antibody (ab95479) at 0.3 µg/ml + MOLT4 lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 100 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
  • ab95479 at 3.8 µg/ml staining NFAT1 in Human spleen by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections. ed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-NFAT1 antibody (ab95479)参考文献

ab95479 has not yet been referenced specifically in any publications.

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