The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
功能Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF.
组织特异性Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas.
序列相似性Contains 1 RHD (Rel-like) domain.
结构域Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
翻译后修饰In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated; which is required for full transcriptional activity.
细胞定位Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
Nuclear factor of activated T cells cytoplasmic 2 antibody
Nuclear factor of activated T cells cytoplasmic calcineurin dependent 2 antibody
Nuclear factor of activated T cells pre-existing component antibody
Nuclear factor of activated T cells, preexisting component antibody
Nuclear factor of activated T-cells antibody
Preexisting nuclear factor of activated T cells 2 antibody
T cell transcription factor NFAT 1 antibody
T-cell transcription factor NFAT1 antibody
Anti-NFAT1 antibody 图像
Western blot - NFATc2 antibody (ab49161)
Anti-NFAT1 antibody (ab49161) at 1.25 µg/ml + SP2/0 cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50,000 - 1/100,000 dilution
Predicted band size : 100 kDa Observed band size : 100 kDa
Anti-NFAT1 antibody (ab49161)参考文献
This product has been referenced in:
Nagamoto-Combs K & Combs CK Microglial phenotype is regulated by activity of the transcription factor, NFAT (nuclear factor of activated T cells). J Neurosci30:9641-6 (2010).
Read more (PubMed: 20631193) »
Heer R et al. Phenotypic modulation of human urinary tract stroma-derived fibroblasts by transforming growth factor beta3. Urology76:509.e13-20 (2010).
Read more (PubMed: 20546875) »