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Synthetic peptide conjugated to KLH derived from within residues 950 - 1050 of Rat Nfasc186.
(Peptide available as ab32637.)
Our Abpromise guarantee covers the use of ab31719 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 186 kDa (predicted molecular weight: 186 kDa).|
Immunohistochemistical detection of Nfasc186 using antibody ab31719 on PFA perfusion-fixed rat brain sections. Primary antibody was diluted at 1/300 and incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: goat anti-rabbit Alexa Fluor 488® diluted (1/1000). Immunostaining observed (image) in the rat cortex. IHC was performed on free-floating sections using direct fluorescence. The antibody produced some staining in the whole CNS.
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