The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 186 kDa (predicted molecular weight: 186 kDa).
Nfasc186 (Neurofascin 186) is one of two alternatively spliced products of the Neurofascin (Nfasc) gene in rat; the other being Nfasc155. The establishment of paranodal axoglial junctions in myelinated nerves where voltage-gated sodium channels are concentrated at the nodes of Ranvier is determined by myelin-forming glia. The two isoforms of Neurofascin, Nfasc186 in neurons and Nfasc155 in glia, are required for the assembly of these specialized domains. These two major Neurofascins play essential roles in assembling the nodal and paranodal domains of myelinated axons and are essential for the transition to saltatory conduction in developing vertebrate nerves. Nfasc186 (Isoform 1) is expressed at the Nodes of Ranvier while the Nfasc155 (isoforms 2 and 3) are expressed in unmyelinated axons.
Western blot - Anti-Nfasc186 antibody - Neuronal Marker (ab31719)
Anti-Nfasc186 antibody - Neuronal Marker (ab31719) at 1 µg/ml + Brain (Rat) Whole Tissue Lysate at 20 µg
Secondary IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 186 kDa Observed band size: 186 kDa
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Nfasc186 antibody - Neuronal Marker (ab31719)This image is courtesy of an abreview submitted by Sophie Pezet, CNRS, Paris, France
Immunohistochemistical detection of Nfasc186 using antibody ab31719 on PFA perfusion-fixed rat brain sections. Primary antibody was diluted at 1/300 and incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: goat anti-rabbit Alexa Fluor 488® diluted (1/1000). Immunostaining observed (image) in the rat cortex. IHC was performed on free-floating sections using direct fluorescence. The antibody produced some staining in the whole CNS.
Martin PM et al. Schwannomin-interacting Protein 1 Isoform IQCJ-SCHIP1 Is a Multipartner Ankyrin- and Spectrin-binding Protein Involved in the Organization of Nodes of Ranvier. J Biol Chem292:2441-2456 (2017).
Read more (PubMed: 27979964) »
Brügger V et al. HDAC1/2-Dependent P0 Expression Maintains Paranodal and Nodal Integrity Independently of Myelin Stability through Interactions with Neurofascins. PLoS Biol13:e1002258 (2015).
Read more (PubMed: 26406915) »