Anti-NF-kB p65 (phospho S276)抗体(ab106129)

概述

  • 产品名称
    Anti-NF-kB p65 (phospho S276)抗体
    参阅全部 NF-kB p65 一抗
  • 描述
    兔多克隆抗体to NF-kB p65 (phospho S276)
  • 特异性
    ab106129 is specific for phosphorylated S276 NF-kB p65. It shows minimal reactivity by Western blot with non-phosphorylated NFkB p65, and minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
  • 经测试应用
    适用于: WB, ELISA, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human NF-kB p65 conjugated to Keyhole Limpet Haemocyanin (KLH). Immunogen = peptide corresponding to a region near phospho Serine 276 of the human protein

  • 阳性对照
    • HeLa whole cell lysates; TNF inducible KBM5 cell lysates.
  • 常规说明

    Dilute only prior to immediate use.
    Expiration date is one (1) year from date of opening.

性能

应用

Our Abpromise guarantee covers the use of ab106129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 60 kDa.
ELISA 1/5000 - 1/25000.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能
    NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.
  • 序列相似性
    Contains 1 RHD (Rel-like) domain.
  • 结构域
    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • 翻译后修饰
    Ubiquitinated, leading to its proteasomal degradation. Degradation is required for termination of NF-kappa-B response.
    Monomethylated at Lys-310 by SETD6. Monomethylation at Lys-310 is recognized by the ANK repeats of EHMT1 and promotes the formation of repressed chromatin at target genes, leading to down-regulation of NF-kappa-B transcription factor activity. Phosphorylation at Ser-311 disrupts the interaction with EHMT1 without preventing monomethylation at Lys-310 and relieves the repression of target genes.
    Phosphorylation at Ser-311 disrupts the interaction with EHMT1 and promotes transcription factor activity (By similarity). Phosphorylation on Ser-536 stimulates acetylation on Lys-310 and interaction with CBP; the phosphorylated and acetylated forms show enhanced transcriptional activity.
    Reversibly acetylated; the acetylation seems to be mediated by CBP, the deacetylation by HDAC3. Acetylation at Lys-122 enhances DNA binding and impairs association with NFKBIA. Acetylation at Lys-310 is required for full transcriptional activity in the absence of effects on DNA binding and NFKBIA association. Acetylation can also lower DNA-binding and results in nuclear export. Interaction with BRMS1 promotes deacetylation of 'Lys-310'.
  • 细胞定位
    Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Colocalized with RELA in the nucleus upon TNF-alpha induction.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Avian reticuloendotheliosis viral (v rel) oncogene homolog A antibody
    • MGC131774 antibody
    • NF kappa B p65delta3 antibody
    • NFKB3 antibody
    • Nuclear Factor NF Kappa B p65 Subunit antibody
    • Nuclear factor NF-kappa-B p65 subunit antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody
    • OTTHUMP00000233473 antibody
    • OTTHUMP00000233474 antibody
    • OTTHUMP00000233475 antibody
    • OTTHUMP00000233476 antibody
    • OTTHUMP00000233900 antibody
    • p65 antibody
    • p65 NF kappaB antibody
    • p65 NFkB antibody
    • relA antibody
    • TF65_HUMAN antibody
    • Transcription factor p65 antibody
    • v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)) antibody
    • V rel avian reticuloendotheliosis viral oncogene homolog A antibody
    • v rel reticuloendotheliosis viral oncogene homolog A (avian) antibody
    • V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65 antibody
    see all

Anti-NF-kB p65 (phospho S276) antibody 图像

  • All lanes : Anti-NF-kB p65 (phospho S276) antibody (ab106129) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysates
    Lane 2 : HeLa whole cell lysates, stimulated with TNF alpha


    Predicted band size : 60 kDa

Anti-NF-kB p65 (phospho S276) antibody (ab106129)参考文献

This product has been referenced in:
  • Nakazawa H  et al. iNOS as a Driver of Inflammation and Apoptosis in Mouse Skeletal Muscle after Burn Injury: Possible Involvement of Sirt1 S-Nitrosylation-Mediated Acetylation of p65 NF-?B and p53. PLoS One 12:e0170391 (2017). WB ; Mouse . Read more (PubMed: 28099528) »
  • Chen RY  et al. Effect of oridonin-mediated hallmark changes on inflammatory pathways in human pancreatic cancer (BxPC-3) cells. World J Gastroenterol 20:14895-903 (2014). WB ; Human . Read more (PubMed: 25356049) »

See all 3 Publications for this product

Product Wall

Application
Western blot
Sample
Rat Cell lysate - whole cell (Primary hippocampal neurons)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Specification
Primary hippocampal neurons
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Sep 30 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (brain)
Specification
brain
Permeabilization
Yes - Triton x-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 26 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (UVB-induced murine skin tissue)
Specification
UVB-induced murine skin tissue
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 37°C
Username

Abcam user community

Verified customer

提交于 Mar 18 2013

Thank you for your reply.
Just to make sure there is no confusion, ab30623 & ab106129 are both NFkB phosphoS276 antibodies, not Re1A. Is this just an alternative name?
If you are looking for an antibody against NFkB phosphoS276, then I would re...

Read More

Thank you for contacting Abcam.
As we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.
We are happy to reassure our you that all of our products are covered by our Abpromise, which guarantees th...

Read More

DISCOUNT CODE: *****
Expiration date: *****

I am very pleased to hear you would like to accept our offer and test ab106129 in IHC and mouse. This code will give you: 1 free primary antibody before the expiration date. To redeem this off...

Read More

Thank you for contacting us.

ab106129 has not been tested in IHC-P or mouse sections, therefore we do not know whether this product is suitable. I can offer a discount off a future purchase if you buy ab106129 now, test it in IHC and mouse a...

Read More
Application
Western blot
Sample
Rat Cell lysate - whole cell (Spinal cord tissue)
Loading amount
20 µg
Specification
Spinal cord tissue
Gel Running Conditions
Reduced Denaturing (4-20% Tris-glycine)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Ms. Kelly Atkins

Verified customer

提交于 Jun 18 2012

DISCOUNT CODE: ***
Expiration date: August 30th, 2012

I am very pleased to hear you would like to accept our offer and test ab106129 in rat. This code will give you: 1 free primary antibody before the expiration date. To redeem this offe...

Read More

Thank you for your inquiry.

I sincerely apologise for the inconvenience the long delay in availability of ab30623 is causing you.

We do offer an alternative antibody that is also specific for the phosphorylation site S276. Unfortuna...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册