Anti-NF-kB p65 (phospho S276)抗体(ab106129)

概述

• 产品名称
  Anti-NF-kB p65 (phospho S276)抗体
  参阅全部 NF-kB p65 一抗
• 描述
  兔多克隆抗体to NF-kB p65 (phospho S276)
• 特异性
  ab106129 is specific for phosphorylated S276 NF-kB p65. It shows minimal reactivity by Western blot with non-phosphorylated NFkB p65, and minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
• 经测试应用
  适用于: WB, ELISA, IHC-Frmore details
• 种属反应性
  与反应: Mouse, Human
  
• 免疫原

  Synthetic peptide corresponding to Human NF-kB p65 conjugated to Keyhole Limpet Haemocyanin (KLH). Immunogen = peptide corresponding to a region near phospho Serine 276 of the human protein

• 阳性对照
  • HeLa whole cell lysates; TNF inducible KBM5 cell lysates.
• 常规说明

  Dilute only prior to immediate use.
  Expiration date is one (1) year from date of opening.

性能

• 形式
  Liquid
• 存放说明
  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
• 存储溶液
  pH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
• Concentration information loading...
• 纯化说明
  ab106129 was prepared from monospecific antiserum by delipidation and defibrination.
• 克隆
  多克隆
• 同种型
  IgG
• 研究领域
  • Cell Biology
  • Apoptosis
  • Intracellular
  • NFkB
  • RelA (p65)

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Other

  • Microbiology
  • Interspecies Interaction
  • Host Virus Interaction

  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • NFkB Pathway

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • NFkB pathway

  • Cancer
  • Signal transduction
  • Nuclear signaling
  • NFkB pathway

  • Cardiovascular
  • Atherosclerosis
  • Vascular Inflammation
  • Inflammatory mediators

  • Microbiology
  • Interspecies Interaction
  • Host Immune Response

  • Metabolism
  • Types of disease
  • Obesity

  • Cancer
  • Cell Death
  • Apoptosis
  • Apoptotic Markers
  • NFkB
  • RelA (p65)

相关产品

• Assay kits
  • NFkB p65 Transcription Factor Assay Kit (ab133112)
• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
• Positive Controls
  • HeLa nuclear extract lysate (ab14655)
  • HeLa whole cell lysate (ab29545)
• Related Products
  • NF kappaB p65 (pS536) ELISA Kit (ab176647)
  • NF kappaB p65 (pS536 + Total) ELISA Kit (ab176663)

应用

靶标

• 功能
  NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.
• 序列相似性
  Contains 1 RHD (Rel-like) domain.
• 结构域
  the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
• 翻译后修饰
  Ubiquitinated, leading to its proteasomal degradation. Degradation is required for termination of NF-kappa-B response.
  Monomethylated at Lys-310 by SETD6. Monomethylation at Lys-310 is recognized by the ANK repeats of EHMT1 and promotes the formation of repressed chromatin at target genes, leading to down-regulation of NF-kappa-B transcription factor activity. Phosphorylation at Ser-311 disrupts the interaction with EHMT1 without preventing monomethylation at Lys-310 and relieves the repression of target genes.
  Phosphorylation at Ser-311 disrupts the interaction with EHMT1 and promotes transcription factor activity (By similarity). Phosphorylation on Ser-536 stimulates acetylation on Lys-310 and interaction with CBP; the phosphorylated and acetylated forms show enhanced transcriptional activity.
  Reversibly acetylated; the acetylation seems to be mediated by CBP, the deacetylation by HDAC3. Acetylation at Lys-122 enhances DNA binding and impairs association with NFKBIA. Acetylation at Lys-310 is required for full transcriptional activity in the absence of effects on DNA binding and NFKBIA association. Acetylation can also lower DNA-binding and results in nuclear export. Interaction with BRMS1 promotes deacetylation of 'Lys-310'.
• 细胞定位
  Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Colocalized with RELA in the nucleus upon TNF-alpha induction.

• Target information above from: UniProt accession Q04206 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 数据库链接
  • Entrez Gene: 5970 Human
  • Entrez Gene: 19697 Mouse
  • Omim: 164014 Human
  • SwissProt: Q04206 Human
  • SwissProt: Q04207 Mouse
  • Unigene: 502875 Human
  • Unigene: 249966 Mouse
• 别名
  • Avian reticuloendotheliosis viral (v rel) oncogene homolog A antibody
  • MGC131774 antibody
  • NF kappa B p65delta3 antibody

  • NFKB3 antibody
  • Nuclear Factor NF Kappa B p65 Subunit antibody
  • Nuclear factor NF-kappa-B p65 subunit antibody
  • Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody
  • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody
  • OTTHUMP00000233473 antibody
  • OTTHUMP00000233474 antibody
  • OTTHUMP00000233475 antibody
  • OTTHUMP00000233476 antibody
  • OTTHUMP00000233900 antibody
  • p65 antibody
  • p65 NF kappaB antibody
  • p65 NFkB antibody
  • relA antibody
  • TF65_HUMAN antibody
  • Transcription factor p65 antibody
  • v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)) antibody
  • V rel avian reticuloendotheliosis viral oncogene homolog A antibody
  • v rel reticuloendotheliosis viral oncogene homolog A (avian) antibody
  • V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65 antibody

  see all

实验方案

• Immunohistochemistry protocols
• Western blot protocols