Neutrophil Elastase Inhibitor Screening试剂盒(ab118971)

概述

  • 产品名称Neutrophil Elastase Inhibitor Screening试剂盒
    参阅全部 Neutrophil Elastase 试剂盒
  • 测试
    100 x 1 assay
  • 检测类型Quantitative
  • 检测时间
    0h 40m
  • 产品概述

    In Abcam's Neutrophil Elastase Inhibitor Screening Kit, NE hydrolyzes a specific fluorescent substrate to release the fluorescent group, which can be detected at Ex/Em = 400/505 nm. In presence of a potent Neutrophil Elastase inhibitor, the hydrolyzation of substrate will be inhibited or stopped. The kit provides a rapid, simple, sensitive, and reliable test suitable as a high throughput screening assay of Neutrophil Elastase inhibitors. For comparison of the relative efficacy of test inhibitors, a control inhibitor, SPCK (Ki = 10 µM for human leukocyte elastase) is included.
    Visit our FAQs page for tips and troubleshooting.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 相关性Neutrophil Elastase (NE) is an aggressive and cytotoxic 29 kDa serine protease stored mainly in the azurophil granules of neutrophil granulocytes. It plays a role in the degradation of a wide range of extracellular matrix proteins, including fibronectin, laminin, proteoglycans, collagens, and elastin. When the extracellular NE concentration exceeds the buffering capacity of endogenous inhibitors, it becomes implicated in the signs, symptoms and disease progression of inflammatory lung disorders via its role in the inflammatory process, mucus overproduction and lung tissue damage.
  • 别名
    • Bone marrow serine protease
    • ELA2
    • ELANE
    • Elastase 2
    • Elastase 2 neutrophil
    • Elastase neutrophil expressed
    • GE
    • Granulocyte derived elastase
    • HLE
    • HNE
    • Human leukocyte elastase
    • Leukocyte elastase
    • Medullasin
    • NE
    • PMN E
    • PMN elastase
    • Polymorphonuclear elastase
    • SCN1
    see all

应用

Our Abpromise guarantee covers the use of ab118971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration.

Neutrophil Elastase Inhibitor Screening Kit 图像

  • Neutrophil Elastase Activity Profile at Various Concentrations of Elafin/Skalp (ab153768)

  • Neutrophil Elastase Activity Profile at Various Concentrations of SERPINB1 (ab114774)

  • Neutrophil Elastase Inhibition by SPCK
  • Neutrophil Elastase Activity Profile at Various Concentrations of SPCK

实验方案

Neutrophil Elastase Inhibitor Screening Kit (ab118971)参考文献

ab118971 has not yet been referenced specifically in any publications.

Product Wall

RFU is an arbitrary unit and is dependent on the specific instrument and the gain/sensitivity, so unfortunately there is no way to give you a generic RFU to nmole correlation for calculation.

1. From what species is the elastase purified? Human.
2. What is the concentration of elastase provided? There is 15-22 mU of enzyme in the kit. The exact amount is proprietary.
3. What is the substrate? Eg. what is the sequence? MeOSuc - Ala -...

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Yes. ab142178 is same as the substrate in ab118971.

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The substrate that is in the kit will be cleaved by active elastase, whether pure, as provided in the kit, or in biological samples. You could use the pure elastase in the kit as a standard for comparison to your...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"