Anti-Neutrophil Elastase抗体(ab68672)

概述

  • 产品名称Anti-Neutrophil Elastase抗体
    参阅全部 Neutrophil Elastase 一抗
  • 描述
    兔多克隆抗体to Neutrophil Elastase
  • 经测试应用适用于: ICC/IF, WB, IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Horse, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Neutrophil Elastase.

    (Peptide available as ab68671.)

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Human Bone Marrow and Mouse Bone Marrow. IHC/P: Human spleen (FFPE)

性能

应用

Our Abpromise guarantee covers the use of ab68672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200. (See abreview 24266)
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 29 kDa).Can be blocked with Human Neutrophil Elastase peptide (ab68671). Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
IHC-Fr Use at an assay dependent concentration. PubMed: 22266908
IHC-P 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.
  • 组织特异性Bone marrow cells.
  • 疾病相关Defects in ELANE are a cause of cyclic haematopoiesis (CH) [MIM:162800]; also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency.
    Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1) [MIM:202700]. SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections.
  • 序列相似性Belongs to the peptidase S1 family. Elastase subfamily.
    Contains 1 peptidase S1 domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Bone marrow serine protease antibody
    • ELA2 antibody
    • ELANE antibody
    • Elastase 2 antibody
    • Elastase 2 neutrophil antibody
    • Elastase neutrophil expressed antibody
    • Elastase-2 antibody
    • ELNE_HUMAN antibody
    • GE antibody
    • Granulocyte derived elastase antibody
    • HLE antibody
    • HNE antibody
    • Human leukocyte elastase antibody
    • Leukocyte elastase antibody
    • Medullasin antibody
    • NE antibody
    • Neutrophil elastase antibody
    • PMN E antibody
    • PMN elastase antibody
    • Polymorphonuclear elastase antibody
    • SCN1 antibody
    see all

Anti-Neutrophil Elastase antibody 图像

  • IHC image of Neutrophil Elastase staining in human spleen formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68672, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Lanes 1 - 2 : Anti-Neutrophil Elastase antibody (ab68672) at 1 µg/ml
    Lanes 3 - 4 : Anti-Neutrophil Elastase antibody (ab68672) at 1 µg/ml (Milk blocking has been used)

    Lane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Mouse Bone Marrow Tissue Lysate
    Lane 3 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
    Lane 4 : Mouse Bone Marrow Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • Immunofluorescent staining with ab68672 of blood film of trangenic mouse that suffered from systemic inflamation. Neutrophil Elastase (in green) expressed in the lysosomes of activated neutrophils. Cells were fixed with methanol and permeabilized with 0.3% TritonX in 0.1% PBS. Blocking was performed with 10% serum for 15 minutes at 24ºC. This was followed by 1 hour incubation at 24ºC with ab68672 which was used at 1/200. The secondary antibody used was an anti-rabbit Alexa Fluor 488.

    See Abreview

  • ab68672 staining Neutrophil Elastase in Mouse LPS treated Lung tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes. Samples were incubated with primary antibody (1/50) for 2 hours. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistical detection of Neutrophil Elastase using antibody (ab68672) on formaldehyde-fixed paraffin-embedded human spleen sections. Antigen retrieval step: heat mediated in citric acid pH6 buffer. Permeabilization: No Blocking step: 1% BSA for 10 mins @ rt°C. Primary antibody dilution 1/2000; incubation time: 2 hours in TBS/BSA/azide. Secondary Antibody: anti Rabbit IgG conjugated to biotin (1/200).

    See Abreview

  • Anti-Neutrophil Elastase antibody (ab68672) at 1 µg/ml + Natural human Neutrophil Elastase protein (ab91099) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa


    Exposure time : 2 minutes

Anti-Neutrophil Elastase antibody (ab68672)参考文献

This product has been referenced in:
  • Zenaro E  et al. Neutrophils promote Alzheimer's disease-like pathology and cognitive decline via LFA-1 integrin. Nat Med N/A:N/A (2015). IF, IHC ; Mouse, Human . Read more (PubMed: 26214837) »
  • Presicce P  et al. Neutrophil recruitment and activation in decidua with intra-amniotic IL-1beta in the preterm rhesus macaque. Biol Reprod 92:56 (2015). Read more (PubMed: 25537373) »

See all 14 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (nasal mucosa)
Permeabilization No
Specification nasal mucosa
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative Paraformaldehyde
Username

Mr. Duc Dung Le

Verified customer

提交于 Jun 08 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH 6.0
Permeabilization No
Specification Lung
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1%
Fixative Formaldehyde
Username

Mr. Simon Cleary

Verified customer

提交于 May 09 2016

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (LPS-treated lung)
Specification LPS-treated lung
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1%
Fixative Formaldehyde
Username

Mr. Simon Cleary

Verified customer

提交于 Dec 02 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rhesus monkey Tissue sections (Colon)
Antigen retrieval step Heat mediated
Permeabilization Yes - 0.1% Triton-X-100
Specification Colon
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Z-FIX
Username

Abcam user community

Verified customer

提交于 Oct 07 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application IHC - Wholemount
Sample Mouse Tissue (Colon)
Specification Colon
Username

Abcam user community

Verified customer

提交于 May 04 2015

The application IHC-Fr in mouse was added for two different reasons for these antibodies. ab21595 was validated for use in mouse in IHC-Fr following an abreview and the images were visualised using a secondary antibody conjugated to Alexa Fluor 594. ab...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cow Tissue sections (Brain of cattle with neutrophil infiltrates)
Specification Brain of cattle with neutrophil infiltrates
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Nov 22 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Cow Cell (Cytospins of bovine neutrophils)
Specification Cytospins of bovine neutrophils
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X 20 min.at room temperature
Username

Abcam user community

Verified customer

提交于 Nov 22 2012

Vielen Dank für Ihre Email.

Frau Zeeck ist in Ihren wohlverdienten Urlaub und deshalb muss ich Ihnen heute die schlechte Nachricht überbringen, dass wir keine anderen Antikörper haben, die bovine Neutrophile Elastase erkennen. ...

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Thank you for contacting us and for sending the incorrect product back to us. I do wish to apologize for the inconvenience this has caused.

According to my information, ab68672 Anti-Neutrophil Elastase antibody is in stock in all of our of...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"