Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.
Bone marrow cells.
Defects in ELANE are a cause of cyclic haematopoiesis (CH) [MIM:162800]; also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency. Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1) [MIM:202700]. SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections.
Belongs to the peptidase S1 family. Elastase subfamily. Contains 1 peptidase S1 domain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil Elastase antibody (ab21595)Image from Oh JY et al, Proc Natl Acad Sci U S A. 2010 Sep 28;107(39):16875-80. Epub 2010 Sep 13, Fig 6. DOI 10.1073/pnas.1012451107
ab21595 staining Neutrophil Elastase in rat cornea tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The section was deparaffinized with ethanol and the antigen was retrieved using a steamer. ab21595 was used at a 1/200 dilution, the secondary was an anti-rabbit IgG 1/5,000 also from Abcam. The DAB staining was performed using a DAB Peroxidase Substrate kit. The slides were counterstained with hematoxylin.
ab21595 staining neutrophil elastase (NE, green), DNA stained with propidium iodide (PI, red) in COPD sputum by ICC/IF (Immunocytochemistry/immunofluorescence). The sputum matrix contains condensed matrix traversed by thin neutrophil extracellular traps (NETs) strands associated with bacteria (arrowheads), non-activated polymorphonulear neutrophils (PMNs) (open arrow) and NETotic PMNs (solid arrow); asterisks indicate fully spread NETs.Cells were blocked with 10% normal goat seum in PBS containing 10 mM glycine and 0.2% Tween 20. Samples were incubated with primary antibody (1/50). ab96883 (1/100) was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil Elastase antibody (ab21595)This image is courtesy of an Abreview submitted by Mr John-Paul Jukes
ab21595 at 1/20 dilution staining rat liver tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The rat was challenged with LPS at 100ug/Kg i.p. The tissue sections were formaldehyde fixed and incubated with the antibody for 2 hours. A biotinylated goat anti-rabbit IgG antibody was used as the secondary antibody.
Herrmann JB et al. Complement C5a Receptor 1 Exacerbates the Pathophysiology ofN. meningitidisSepsis and Is a Potential Target for Disease Treatment. MBio9:N/A (2018).
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Carmona-Rivera C et al. A role for muscarinic receptors in neutrophil extracellular trap formation and levamisole-induced autoimmunity. JCI Insight2:e89780 (2017).
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