Anti-Neutrophil抗体[NIMP-R14] (Phycoerythrin) (ab125159)

概述

  • 产品名称Anti-Neutrophil抗体[NIMP-R14] (Phycoerythrin)
    参阅全部 Neutrophil 一抗
  • 描述
    大鼠单克隆抗体[NIMP-R14] to Neutrophil (Phycoerythrin)
  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm
  • 经测试应用适用于: IHC-P, IHC-Fr, Functional Studies, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Purified BALB/c Mouse neutrophils.

  • 表位The monoclonal antibody NIMP-R14 is highly specific for murine Ly-6G and Ly-6C.
  • 阳性对照
    • Mouse spleen tissue; Mouse neutrophils.
  • 常规说明

     

     

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.02% Sodium azide
    Constituents: 98% PBS, 1% BSA
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号NIMP-R14
  • 同种型IgG2b
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab125159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. Blocking with 20% normal rabbit serum.
IHC-Fr Use at an assay dependent concentration. Tissue was fixed with acetone.
Functional Studies Use at an assay dependent concentration.
Flow Cyt Use 10µl for 105 cells. ab136585-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 23109724

靶标

  • 细胞定位Cell membrane; Lipid-anchor, GPI-anchor
  • 数据库链接
  • 别名
    • Ly-6B antibody
    • Ly6b antibody
    • Lymphocyte antigen 6B antibody
    • Lymphocyte antigen 6B Short name=Ly-6B antibody

Anti-Neutrophil antibody [NIMP-R14] (Phycoerythrin) (ab125159)参考文献

This product has been referenced in:
  • Menten-Dedoyart C  et al. Neutrophil extracellular traps entrap and kill Borrelia burgdorferi sensu stricto spirochetes and are not affected by Ixodes ricinus tick saliva. J Immunol 189:5393-401 (2012). ICC/IF ; Mouse . Read more (PubMed: 23109724) »
  • de Vries B  et al. Complement factor C5a mediates renal ischemia-reperfusion injury independent from neutrophils. J Immunol 170:3883-9 (2003). Read more (PubMed: 12646657) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (hepatocytes)
Permeabilization No
Specification hepatocytes
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative Paraformaldehyde
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提交于 Aug 09 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"