大鼠单克隆抗体[NIMP-R14] to Neutrophil (Phycoerythrin)
Phycoerythrin. Ex: 488nm, Em: 575nm
ICC/IF more details
Purified BALB/c Mouse neutrophils.
The monoclonal antibody NIMP-R14 is highly specific for murine Ly-6G and Ly-6C.
Mouse spleen tissue; Mouse neutrophils.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Blocking with 20% normal rabbit serum.
Use at an assay dependent concentration. Tissue was fixed with acetone.
Use at an assay dependent concentration.
Use 10µl for 10
5 cells. ab136585-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration. PubMed: 23109724
Anti-Neutrophil antibody [NIMP-R14] (Phycoerythrin) 图像
Flow Cytometry - Anti-Neutrophil antibody [NIMP-R14] (Phycoerythrin) (ab125159)
Detection of Ly-6G/-6C in RAW cells. Red, black and blue line represent the isotype control, cells only and ab125159 at 10 μg/ml, respectively.
Anti-Neutrophil antibody [NIMP-R14] (Phycoerythrin) (ab125159)参考文献
This product has been referenced in:
et al. Neutrophil extracellular traps entrap and kill Borrelia burgdorferi sensu stricto spirochetes and are not affected by Ixodes ricinus tick saliva. J Immunol 189:5393-401 (2012).
Read more (PubMed: 23109724) »
de Vries B
et al. Complement factor C5a mediates renal ischemia-reperfusion injury independent from neutrophils. J Immunol 170:3883-9 (2003).
Read more (PubMed: 12646657) »
See all 4 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"