Anti-Neutrophil抗体[NIMP-R14] (ab2557)

概述

  • 产品名称
    Anti-Neutrophil抗体[NIMP-R14]
    参阅全部 Neutrophil 一抗
  • 描述
    大鼠单克隆抗体[NIMP-R14] to Neutrophil
  • 经测试应用
    适用于: ICC, Functional Studies, IHC-P, Flow Cyt, IHC-Frmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Tissue, cells or virus corresponding to Mouse Neutrophil. Purified BALB/c mouse neutrophils

  • 表位
    The monoclonal antibody NIMP-R14 is highly specific for murine Ly-6G and Ly-6C.
  • 常规说明

    The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly-6G is together with Ly-6C a component of the myeloid differentiation antigen Gr-1. Ly-6G is a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating premonocytes and plasmacytoid dendritic cells. Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly-6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    PBS , 0.1% BSA and 0.02% sodium azide
  • Concentration information loading...
  • 纯度
    IgG fraction
  • Primary antibody说明
    The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly6G is together with Ly6c a component of the myeloid differentiation antigen Gr-1. Ly6G a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating premonocytes and plasmacytoid dendritic cells. Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.
  • 克隆
    单克隆
  • 克隆编号
    NIMP-R14
  • 骨髓瘤
    210RCY3-Ag123
  • 同种型
    IgG2b
  • 轻链类型
    unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab2557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration. Neutrophil depletion. Mice were treated with NIMP-R14 given intraperitoneally at a dose of 1 mg, 6 hours before infection.
IHC-P Use at an assay dependent concentration.

This antibody has been reported to work well without antigen retrieval, or with retrieval in a 125 degree C decloaking chamber for 3 minutes with pH 6.5 buffer. HIER optimization may be required.

Flow Cyt Use at an assay dependent concentration.

5 x 105 cells were incubated with 10 µg/ml antibody.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration. Tested with acetone-fixed tissue.

靶标

  • 细胞定位
    Cell membrane; Lipid-anchor, GPI-anchor
  • 数据库链接
  • 别名
    • Ly-6B antibody
    • Ly6b antibody
    • Lymphocyte antigen 6B antibody
    • Lymphocyte antigen 6B Short name=Ly-6B antibody

Anti-Neutrophil antibody [NIMP-R14] 图像

  • Frozen sections of mouse spleen. ab2557 was used in a concentration of 5 μg/ml.

  • ab2557 at 1/80 staining mouse blood cells (ip wash cells) by Immunocytochemistry. The antibody was incubated with the cells for 1 hour and then detected using a biotinylated rabbit anti-rat (mouse adsorbed) antibody.

    This image is courtesy of an Abreview submitted on 16 March 2006.

    See Abreview

  • ab2557 staining Neutrophil in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formalin and blocked with 2.5% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in 10mM sodium citrate. Samples were incubated with primary antibody (1/200 in 1x PBS/1% BSA/0.3% Triton X-100 buffer) for 1 hour at 25°C. A HRP-conjugated Goat anti-rat Ig polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • ab2557 staining Ly-6G/-6C in mouse spleen tissue by Immunohistochemistry (Frozen sections).
  • ab2557 staining Neutrophil in mouse tumor tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then blocked, followed by incubation with the primary antibody at a 1/50 dilution for 30 minutes. A biotin-conjugated rabbit anti-rat was used as secondary antibody at a 1/400 dilution.

Anti-Neutrophil antibody [NIMP-R14] (ab2557)参考文献

This product has been referenced in:
  • Kong Q  et al. HSPA12B Attenuated Acute Myocardial Ischemia/reperfusion Injury via Maintaining Endothelial Integrity in a PI3K/Akt/mTOR-dependent Mechanism. Sci Rep 6:33636 (2016). Mouse . Read more (PubMed: 27644317) »
  • Hasan AS  et al. Cardiosphere-Derived Cells Facilitate Heart Repair by Modulating M1/M2 Macrophage Polarization and Neutrophil Recruitment. PLoS One 11:e0165255 (2016). Read more (PubMed: 27764217) »

See all 57 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TE pH9
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 18 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Citrate
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
Username

Dr. Wesley Konsavage

Verified customer

提交于 Dec 22 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Skin)
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
Username

Dr. Victoria Thompson

Verified customer

提交于 Jun 23 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ph6 citrate
Permeabilization
No
Specification
Skin
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
Username

Dr. Victoria Thompson

Verified customer

提交于 Jun 23 2016

Application
Flow Cytometry
Fixation
None, cells were used fresh
Permeabilization
No
Sample
Mouse Cell (Cells from bronchoaveolar lavage in balb/c mice)
Specification
Cells from bronchoaveolar lavage in balb/c mice
Gating Strategy
alive (DAPI-),CD45+, C11b+, CD11c- cells
Preparation
Cell harvesting/tissue preparation method: Bronchoaveolar lavage in balb/c mice was performed with PBS-EDTA 3mM
Sample buffer: PBS-EDTA 3mM
Username

Dr. Francois Daubeuf

Verified customer

提交于 Feb 20 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM sodium citrate
Sample
Mouse Tissue sections (lung)
Specification
lung
Permeabilization
No
Fixative
Formalin
Username

Abcam user community

Verified customer

提交于 Jan 02 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Sample
Mouse Tissue sections (brain)
Specification
brain
Permeabilization
Yes - Triton-X
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 05 2013

Thank you for contacting us.

Unfortunately, we cannot test every organ or tissue when we establish a product.However, if the knee joints were decalcified properly and the species is tested, I don't see any problems why these antibodies shoul...

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Thank you for contacting us.

We have tested this antibody with PFA fixed paraffin embedded tissue section and one of our customer have submitted positive Abreview for Immunohistochemistry on frozen sections using acetone fixative so it seems t...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (blood)
Specification
blood
Fixative
Paraformaldehyde
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Trypsin
Permeabilization
Yes - Triton X
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Kate Schunke

Verified customer

提交于 Oct 02 2012

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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