Anti-Neutrophil抗体[7/4] (FITC) (ab53453)

概述

  • 产品名称
    Anti-Neutrophil抗体[7/4] (FITC)
    参阅全部 Neutrophil 一抗
  • 描述
    大鼠单克隆抗体[7/4] to Neutrophil (FITC)
  • 偶联物
    FITC. Ex: 493nm, Em: 528nm
  • 特异性
    Rat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) strains. A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity.
  • 经测试应用
    适用于: IHC-FoFr, IHC-Fr, Flow Cytmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Tissue/ cell preparation: cultured bone marrow cells.

  • 表位
    Recognizes the Ly-6B.2 antigen.
  • 阳性对照
    • Flow Cyt.: New Zealand Black mouse peripheral blood granulocytes.
  • 常规说明

    ab53453 recognizes the Ly-6B.2 antigen. Ly-6B.2 is a ~25-30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages. High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15 kDa.

    ab53453 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow and the depletion of myeloid cells in vivo.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 纯化说明
    Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
  • Primary antibody说明
    ab53453 recognises a neutrophil allotypic marker, a polymorphic 40kD antigen expressed by polymorphonuclear cells, but absent on resident tissue macrophages.
  • 克隆
    单克隆
  • 克隆编号
    7/4
  • 骨髓瘤
    Y3/Ag1.2.3
  • 同种型
    IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab53453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration. PubMed: 20038794
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Use 10µl of the working dilution to label 106 cells in 100µl.
ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 细胞定位
    Cell membrane; Lipid-anchor, GPI-anchor
  • 数据库链接
  • 别名
    • Ly-6B antibody
    • Ly6b antibody
    • Lymphocyte antigen 6B antibody
    • Lymphocyte antigen 6B Short name=Ly-6B antibody

Anti-Neutrophil antibody [7/4] (FITC) 图像

  • Immunohistochemical analysis of acetone-fixed frozen mouse spleen, labelling Neutrophil with ab53453 at a dilution of 1/100 incubated for 1 hour at 25°C in 5% goat serum in TBS. The secondary used (to amplify the signal) was a Goat anti-rat polyclonal Alexa Fluor® 488 conjugate at a 1/400 dilution.

    See Abreview

  • Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti mouse neutrophil:FITC (ab53453).

Anti-Neutrophil antibody [7/4] (FITC) (ab53453)参考文献

This product has been referenced in:

See all 11 Publications for this product

Product Wall

Swiss PO is used to denote "Swiss-Pathology Oxford," a specific strain at Oxford University.

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Dog Cell (from whole blood)
Specification
from whole blood
Preparation
Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
alive neutrophils
Username

Abcam user community

Verified customer

提交于 Nov 12 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Cynomolgus Monkey Cell (Whole blood)
Specification
Whole blood
Preparation
Cell harvesting/tissue preparation method: After red blood cell lysis
Sample buffer: PBS/BSA 1%/Azide 0.01%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
alive granulocytes
Username

Abcam user community

Verified customer

提交于 Apr 27 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Rat Cell (Splenocytes)
Specification
Splenocytes
Preparation
Cell harvesting/tissue preparation method: spleen was mashed and red blood cells lysed
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization
No
Gating Strategy
alive monocytes
Username

Abcam user community

Verified customer

提交于 Apr 27 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Spleen from immune mouse)
Permeabilization
No
Specification
Spleen from immune mouse
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Acetone
Username

Abcam user community

Verified customer

提交于 Apr 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Mouse Cell (cells from blood)
Specification
cells from blood
Preparation
Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Permeabilization
No
Gating Strategy
neutrophils
Username

Abcam user community

Verified customer

提交于 Dec 20 2011

Thank you for your enquiry. I am pleased to confirm the following information: ab105157 Datasheet suggests 10 ul per test 50 tests = 500 ul ab53453 On the datasheet, provide 50 ug at 0.1 mg/ml = 500ul I hope ...

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Thank you for contacting us. Please check the following catalogue number; ab53457 ab53453 ab105157 ab2557 These are exact match to your requirements however I would recommend reading the datasheets carefully and buying t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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