FITC荧光Anti-Neutrophil抗体[7/4] (ab53453)
Key features and details
- FITC Rat monoclonal [7/4] to Neutrophil
- Suitable for: IHC-FoFr, IHC-Fr, Flow Cyt, IHC-P
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Related conjugates and formulations
概述
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产品名称
FITC荧光Anti-Neutrophil抗体[7/4]
参阅全部 Neutrophil 一抗 -
描述
FITC荧光大鼠单克隆抗体[7/4] to Neutrophil -
宿主
Rat -
偶联物
FITC. Ex: 493nm, Em: 528nm -
特异性
Rat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) strains. A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity. -
经测试应用
适用于: IHC-FoFr, IHC-Fr, Flow Cyt, IHC-Pmore details -
种属反应性
与反应: Mouse -
免疫原
Tissue, cells or virus corresponding to Mouse Neutrophil. Cultured bone marrow cells.
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表位
Recognizes the Ly-6B.2 antigen. -
阳性对照
- Flow Cyt.: New Zealand Black mouse peripheral blood granulocytes.
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常规说明
ab53453 recognizes the Ly-6B.2 antigen. Ly-6B.2 is a ~25-30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages. High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15 kDa.
ab53453 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow and the depletion of myeloid cells in vivo.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. -
Primary antibody说明
ab53453 recognises a neutrophil allotypic marker, a polymorphic 40kD antigen expressed by polymorphonuclear cells, but absent on resident tissue macrophages. -
克隆
单克隆 -
克隆编号
7/4 -
骨髓瘤
Y3/Ag1.2.3 -
同种型
IgG2a -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53453于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-FoFr |
Use at an assay dependent concentration. PubMed: 20038794
|
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IHC-Fr | (1) |
Use at an assay dependent concentration.
|
Flow Cyt | (4) |
Use at an assay dependent concentration.
Use 10µl of the working dilution to label 106 cells in 100µl. |
IHC-P |
Use at an assay dependent concentration.
|
说明 |
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IHC-FoFr
Use at an assay dependent concentration. PubMed: 20038794 |
IHC-Fr
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. Use 10µl of the working dilution to label 106 cells in 100µl. |
IHC-P
Use at an assay dependent concentration. |
靶标
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细胞定位
Cell membrane; Lipid-anchor, GPI-anchor -
数据库链接
- Entrez Gene: 110453 Mouse
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别名
- Ly-6B antibody
- Ly6b antibody
- Lymphocyte antigen 6B antibody
- Lymphocyte antigen 6B Short name=Ly-6B antibody
图片
实验方案
数据表及文件
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Datasheet download
文献 (23)
ab53453 被引用在 23 文献中.
- Moreira D et al. Myeloid cell-targeted STAT3 inhibition sensitizes head and neck cancers to radiotherapy and T cell-mediated immunity. J Clin Invest 131:N/A (2021). PubMed: 33232304
- Reglero-Real N et al. Autophagy modulates endothelial junctions to restrain neutrophil diapedesis during inflammation. Immunity 54:1989-2004.e9 (2021). PubMed: 34363750
- Kang L et al. Neutrophil extracellular traps released by neutrophils impair revascularization and vascular remodeling after stroke. Nat Commun 11:2488 (2020). PubMed: 32427863
- Ma F et al. Endothelium-targeted deletion of the miR-15a/16-1 cluster ameliorates blood-brain barrier dysfunction in ischemic stroke. Sci Signal 13:N/A (2020). PubMed: 32265338
- Fox JM et al. Optimal therapeutic activity of monoclonal antibodies against chikungunya virus requires Fc-Fc?R interaction on monocytes. Sci Immunol 4:N/A (2019). PubMed: 30796092