Anti-Neutrophil抗体[7/4] (ab53457)

概述

  • 产品名称
    Anti-Neutrophil抗体[7/4]
    参阅全部 Neutrophil 一抗
  • 描述
    大鼠单克隆抗体[7/4] to Neutrophil
  • 特异性
    Rat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) strains. A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity.
  • 经测试应用
    适用于: IHC-P, ICC/IF, WB, IHC-Fr, Flow Cytmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Tissue, cells or virus corresponding to Neutrophil.

  • 表位
    Recognizes the Ly-6B.2 antigen.
  • 阳性对照
    • Flow cytometry: Mouse peripheral blood granulocytes.
  • 常规说明

    Ly-6B.2 is a ~25-30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages. High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15 kDa.

    ab53457 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow and the depletion of myeloid cells in vivo.

     

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 纯化说明
    Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
  • 克隆
    单克隆
  • 克隆编号
    7/4
  • 骨髓瘤
    Y3/Ag1.2.3
  • 同种型
    IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab53457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. PubMed: 25505239
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt 1/50 - 1/100. Use 10µl of the suggested working dilution to label 106 cells in 100µl.



ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 细胞定位
    Cell membrane; Lipid-anchor, GPI-anchor
  • 数据库链接
  • 别名
    • Ly-6B antibody
    • Ly6b antibody
    • Lymphocyte antigen 6B antibody
    • Lymphocyte antigen 6B Short name=Ly-6B antibody

Anti-Neutrophil antibody [7/4] 图像

  • Staining of mouse peripheral blood granulocytes with Rat anti mouse neutrophil (ab53457).

Anti-Neutrophil antibody [7/4] (ab53457)参考文献

This product has been referenced in:
  • Amrouche L  et al. MicroRNA-146a in Human and Experimental Ischemic AKI: CXCL8-Dependent Mechanism of Action. J Am Soc Nephrol 28:479-493 (2017). Read more (PubMed: 27444565) »
  • Puccini JM  et al. Leucine-rich repeat kinase 2 modulates neuroinflammation and neurotoxicity in models of human immunodeficiency virus 1-associated neurocognitive disorders. J Neurosci 35:5271-83 (2015). IHC ; Mouse . Read more (PubMed: 25834052) »

See all 7 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 12 2016

Thank you for contacting us this afternoon and reporting the problems encountered with the most recent lot of the Anti-Neutrophil antibody [7/4] (ab53457). I am sorry to hear this antibody is not providing satisfactory results.

As discussed ...

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Thank you for contacting us.

This product has not been tested in that particular strain. However it is covered by our Abpromise and guaranteed to work on mouse in any of the tested applications listed on the datasheet.

I hope this...

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Thank you for contacting us.

Thank you for your inquiry. We have not tested MCA771GA on the Vm/Dk mouse strain. We ran a literature search, butwere unable to find any publications in which customers have tried this antibody on this mouse str...

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Thank you for contacting us. Please check the following catalogue number; ab53457 ab53453 ab105157 ab2557 These are exact match to your requirements however I would recommend reading the datasheets carefully and buying t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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