Anti-Neurturin抗体(ab63949)

概述

  • 产品名称Anti-Neurturin抗体
    参阅全部 Neurturin 一抗
  • 描述
    山羊多克隆抗体to Neurturin
  • 经测试应用适用于: IHC-Fr, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide:

    DVHSRYHTLQELSARE

    , corresponding to C terminal amino acids 176-191 of Mouse Neurturin

  • 阳性对照
    • Mouse Brain and Rat Brain lysates

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab63949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/200.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 22 kDa.
ELISA 1/64000.

靶标

  • 功能Supports the survival of sympathetic neurons in culture. May regulate the development and maintenance of the CNS. Might control the size of non-neuronal cell population such as haemopoietic cells.
  • 疾病相关Defects in NRTN are a cause of Hirschsprung disease (HSCR) [MIM:142623]. In association with mutations of RET gene, and possibly with other loci, defects in NRTN are involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction.
  • 序列相似性Belongs to the TGF-beta family. GDNF subfamily.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Neurturin antibody
    • Neurturin precursor antibody
    • NRTN antibody
    • NRTN_HUMAN antibody
    • NTN antibody
    • prepro-neurturin antibody
    see all

Anti-Neurturin antibody 图像

  • Anti-Neurturin antibody (ab63949) at 0.1 µg/ml (1 hour) + Mouse Brain lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
  • ab63949 staining Neurturin in Mouse brain tissue by Immunohistochemistry (Frozen sections). The sections were fixed in 4% PFA, followed by 30% sucrose and permeabilized with Triton X-100 prior to blocking with 10% goat serum for 20 minutes at 23°C. The primary antibody was diluted 1/200 in the blocking buffer and incubated with the sample for 90 minutes at 23°C. A biotin-conjugated Rabbit anti-Goat was used as the secondary antibody, diluted 1/200.

Anti-Neurturin antibody (ab63949)参考文献

ab63949 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative 4% PF, followed by 30% sucrose
Permeabilization Yes - Triton X 100
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 23°C
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提交于 Nov 11 2011

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