Anti-Neurotrophin 3抗体(ab65804)

概述

  • 产品名称Anti-Neurotrophin 3抗体
    参阅全部 Neurotrophin 3 一抗
  • 描述
    兔多克隆抗体to Neurotrophin 3
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Neurotrophin 3 aa 174-189 (internal sequence).
    Sequence:

    TVLGEIKTGNSPVKQY


    (Peptide available as ab182403)

  • 阳性对照
    • Rat brain tissue sections and rat brain tissue lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide, 0.01% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, 0.18% Sodium chloride, 0.04% Dibasic monohydrogen sodium phosphate, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab65804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 23236374
WB Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 29 kDa).Can be blocked with Neurotrophin 3 peptide (ab182403).
IHC-P Use a concentration of 0.5 - 1 µg/ml.

Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.

靶标

Anti-Neurotrophin 3 antibody 图像

  • ABC immunocytochemistry of rat dorsal root ganglion neurons with ab65804. The DRG neurons were post-fixed with Zamboni's fixative for 20 minutes.

  • ab65804 at 0.5µg/ml staining Neurotrophin 3 in rat brain tissue sections.
  • All lanes : Anti-Neurotrophin 3 antibody (ab65804) at 2 µg/ml

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat brain tissue lysate
    Lane 3 : MCF7 whole cell lysate
    Lane 4 : HeLa whole cell lysate


    Predicted band size : 29 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)

  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa

    ab9792 = Active human Neurotrophin 3 protein fragment

Anti-Neurotrophin 3 antibody (ab65804)参考文献

This product has been referenced in:
  • Wang H  et al. Reduced neurotrophic factor level is the early event before the functional neuronal deficiency in high-fat diet induced obese mice. Metab Brain Dis N/A:N/A (2016). Read more (PubMed: 27624843) »
  • Wang J  et al. The Effect of Repeated Electroacupuncture Analgesia on Neurotrophic and Cytokine Factors in Neuropathic Pain Rats. Evid Based Complement Alternat Med 2016:8403064 (2016). WB . Read more (PubMed: 27800006) »

See all 9 Publications for this product

Product Wall

Application Western blot
Sample Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 20 µg
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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提交于 Mar 16 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 20 µg
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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提交于 Mar 16 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (tooth)
Antigen retrieval step None
Permeabilization No
Specification tooth
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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提交于 Oct 30 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Dako Flex peroxidase as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: High pH
Sample Human Tissue sections (Tendon)
Specification Tendon
Permeabilization No
Fixative 10% buffered formalin
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提交于 Oct 20 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12% in TGS)
Sample Human Cell lysate - whole cell (Primary astrocytes)
Specification Primary astrocytes
Blocking step Li-Cor Block Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 21°C
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Dr. Grant Corbett

Verified customer

提交于 Jul 25 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (15% Gel)
Sample Rat Tissue lysate - whole (Skeletal muscle)
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Abcam user community

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提交于 Jun 11 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (15% Gel)
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 11 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Recombinant protein (HEK Cell supernatant)
Loading amount 10 µg
Specification HEK Cell supernatant
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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提交于 Mar 12 2013

Thank you for contacting us with your question. I have confirmed that the lab uses normal MCF7 and HeLa cell lysates to test ab65804. I hope this information is helpful, but please let me know if you have any further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"