Anti-Neurotrophin 3抗体(ab53685)

概述

  • 产品名称
    Anti-Neurotrophin 3抗体
    参阅全部 Neurotrophin 3 一抗
  • 描述
    兔多克隆抗体to Neurotrophin 3
  • 特异性
    ab53685 shows reactivity of less than 0.1% with mouse NGF, recombinant human BDNF or Neurotrophin 4.
  • 经测试应用
    适用于: WB, ELISA, Inhibition Assay, IHC-Frmore details
  • 种属反应性
    与反应: Rat, Chicken, Human
  • 免疫原

    Synthetic peptide, corresponding to N terminal amino acids 139-149 of Mouse Neurotrophin 3 conjugated to BSA.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab53685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 30 kDa.
ELISA Use a concentration of 1 - 10 µg/ml.
Inhibition Assay Use a concentration of 1 - 10 µg/ml.
IHC-Fr Use at an assay dependent concentration.

靶标

Anti-Neurotrophin 3 antibody 图像

  • All lanes : Anti-Neurotrophin 3 antibody (ab53685) at 1/500 dilution

    Lane 1 : Rat Brain (Age P21) Whole Tissue ysate
    Lane 2 : Rat Brain (Age P21) Whole Tissue ysate
    Lane 3 : Rat Brain (Age P21) Whole Tissue ysate
    Lane 4 : Rat Brain (Age P21) Whole Tissue ysate

    Secondary
    Dako Cytomation P0260 Conjugation: HRP
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Additional bands at : 32 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

    This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

    See Abreview

Anti-Neurotrophin 3 antibody (ab53685)参考文献

ab53685 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (day 11,5 Embryo)
Permeabilization
No
Specification
day 11,5 Embryo
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Mr. Pierre-Yves Canto

Verified customer

提交于 Aug 29 2016

Thank you for your inquiry. This antibody to NT3 has been tested for biological activity using an in vitro assay based on the culture of embryonic sensory neurons from the chick dorsal root ganglia. The assay is performed according to the reference...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Brain Whole Tissue Lysate)
Loading amount
10 µg
Specification
Brain Whole Tissue Lysate
Gel Running Conditions
Reduced Denaturing (15% Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Dec 21 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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