Anti-Neurotensin Receptor 1抗体(ab75555)

概述

  • 产品名称
    Anti-Neurotensin Receptor 1抗体
    参阅全部 Neurotensin Receptor 1 一抗
  • 描述
    兔多克隆抗体to Neurotensin Receptor 1
  • 特异性
    ab75555 detects endogenous levels of total Neurotensin receptor 1 protein.
  • 经测试应用
    适用于: ICC/IF, WB, Indirect ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthesized peptide derived from an internal sequence of human Neurotensin Receptor 1

  • 阳性对照
    • Extracts from Jurkat cells

性能

应用

Our Abpromise guarantee covers the use of ab75555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 46 kDa.
Indirect ELISA 1/5000.

靶标

  • 相关性
    Neurotensin receptor 1 belongs to the large superfamily of G-protein coupled receptors. NTSR1 mediates the multiple functions of neurotensin, such as hypotension, hyperglycemia, hypothermia, antinociception, and regulation of intestinal motility and secretion. NTR1 has been reported in brain, pancreas, and small intestine. ESTs have been isolated from brain and colon libraries.
  • 细胞定位
    Cell Membrane, Endoplasmic reticulum, Golgi Apparatus and Plasma membrane. Integral membrane protein.
  • 数据库链接
  • 别名
    • High affinity levocabastine insensitive neurotensin receptor antibody
    • Neurotensin receptor 1 (high affinity) antibody
    • Neurotensin receptor type 1 antibody
    • NT R 1 antibody
    • NTR antibody
    • NTR1 antibody
    • NTR1_HUMAN antibody
    • NTRH antibody
    • NTRR antibody
    • NTSR 1 antibody
    see all

图片

  • All lanes : Anti-Neurotensin Receptor 1 antibody (ab75555) at 1/500 dilution

    Lane 1 : extracts from Jurkat cells
    Lane 2 : extracts from Jurkat cells with immunizing peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 46 kDa
    Observed band size : 46 kDa
  • ICC/IF image of ab75555 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75555, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

ab75555 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Loading amount
15 µg
Specification
brain
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 Oct 22 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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