Anti-Neuropeptide Y抗体(ab30914)

Anti-Neuropeptide Y antibody 图像

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody (ab30914)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

  Immunohistochemistical detection of Neuropeptide Y using ab30914 on  formaldehyde-fixed paraffin-embedded rat brain sections. The image shows NPY staining in the cerebellum and frontal cortex of the rat brain. Formaldehyde antigen retrieval step: heat mediated in cittric acid ph6 buffer. Blocking step: 1% BSA for 10 mins at room temperature. ab30914 incubated at 1/1000 for 2 hours in TBS/BSA/azide. Secondary Antibody: anti-rabbit IgG conjugated to biotin (1/200)

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody (ab30914)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

  Immunohistochemistical detection of Neuropeptide Y antibody (ab30914) on formaldehyde fixed paraffin-embedded human foetal wall sectionsAntigen retrieval step: Heat mediated. Buffer: Citric acid pH6. Permeabilization: None.  Blocking step: 1% BSA for 10 mins at 21°C. Primary antibody incubated at 1/1500 for 2 hours at 21°C in TBS/BSA/azide. Abcam's EXPOSE detection kit was used: ab80436. NB: In the submitted image arrowheads indicate positive features: red for ganglion cells in the Submucosa (Meissner's plexus), green for ganglion cells in the Muscularis externa (Auerbach's plexus). There is also a diffuse fine positivity throughout the smooth muscle cells of the externa.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody (ab30914)
  IHC image of Neuropeptide Y staining in human cerebral cortex FFPE section, performed on a Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab30914, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
• 

  Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Neuropeptide Y antibody (ab30914)This image is courtesy of an Abreview submitted by Dr Sophie Pezet

  ab30914 staining Neuropeptide Y in rat brain tissue section by IHC-FoFr (PFA perfusion fixed frozen sections). Rats were perfused with 4% PFA;  tissue was fixed overnight in the same fixative and were cryoprotected in 30% sucrose and cut using a cryostat. The sample was incubated with primary antibody dilution 1/3000 (PBS + 0.3% Triton X100) at 20°C for 18 hours. The Alexa Fluor^® 488 conjugated Goat polyclonal to rabbit IgG dilution 1/1000 was used as secondary antibody. The image shows staining in the soma and processes of neurons with the expected distribution of Neuropeptide Y.

  See Abreview