- 数据表
- 具体的参考文献 (7)
- 实验方案
概述
-
产品名称Anti-Neuropeptide Y抗体
参阅全部 Neuropeptide Y 一抗 -
描述兔多克隆抗体to Neuropeptide Y
-
经测试应用适用于: IHC-P, IHC-FoFrmore details
-
种属反应性与反应: Mouse, Rat, Human, Common marmoset
预测可用于: Sheep, Rabbit, Chicken, Guinea pig, Cow, Pigeon, Pig, Zebrafish, Rhesus monkey
-
免疫原
Synthetic peptide conjugated to KLH derived from within residues 1 to the C-terminus of Pig Neuropeptide Y.
(Peptide available as ab32971.)
-
阳性对照
- IHC-P: Human cerebral cortex tissue.
性能
-
形式Liquid
-
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
-
克隆多克隆
-
同种型IgG
-
研究领域
相关产品
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Immunohistochemistry kits
应用
Our Abpromise guarantee covers the use of ab30914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| IHC-FoFr | 1/3000. |
靶标
-
功能NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.
-
组织特异性One of the most abundant peptides in the nervous system. Also found in some chromaffin cells of the adrenal medulla.
-
序列相似性Belongs to the NPY family.
-
细胞定位Secreted.
- Information by UniProt
-
数据库链接
- Entrez Gene: 396464 Chicken
- Entrez Gene: 504216 Cow
- Entrez Gene: 100724008 Guinea pig
- Entrez Gene: 4852 Human
- Entrez Gene: 109648 Mouse
- Entrez Gene: 397304 Pig
- Entrez Gene: 24604 Rat
- Entrez Gene: 574114 Rhesus monkey
see all -
别名
- C-flanking peptide of NPY antibody
- CPON antibody
- Neuropeptide tyrosine antibody
see all
Anti-Neuropeptide Y antibody 图像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody (ab30914)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United KingdomImmunohistochemistical detection of Neuropeptide Y using ab30914 on formaldehyde-fixed paraffin-embedded rat brain sections. The image shows NPY staining in the cerebellum and frontal cortex of the rat brain. Formaldehyde antigen retrieval step: heat mediated in cittric acid ph6 buffer. Blocking step: 1% BSA for 10 mins at room temperature. ab30914 incubated at 1/1000 for 2 hours in TBS/BSA/azide. Secondary Antibody: anti-rabbit IgG conjugated to biotin (1/200)
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody (ab30914)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United KingdomImmunohistochemistical detection of Neuropeptide Y antibody (ab30914) on formaldehyde fixed paraffin-embedded human foetal wall sectionsAntigen retrieval step: Heat mediated. Buffer: Citric acid pH6. Permeabilization: None. Blocking step: 1% BSA for 10 mins at 21°C. Primary antibody incubated at 1/1500 for 2 hours at 21°C in TBS/BSA/azide. Abcam's EXPOSE detection kit was used: ab80436. NB: In the submitted image arrowheads indicate positive features: red for ganglion cells in the Submucosa (Meissner's plexus), green for ganglion cells in the Muscularis externa (Auerbach's plexus). There is also a diffuse fine positivity throughout the smooth muscle cells of the externa.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody (ab30914)IHC image of Neuropeptide Y staining in human cerebral cortex FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab30914, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Neuropeptide Y antibody (ab30914)This image is courtesy of an Abreview submitted by Dr Sophie Pezetab30914 staining Neuropeptide Y in rat brain tissue section by IHC-FoFr (PFA perfusion fixed frozen sections). Rats were perfused with 4% PFA; tissue was fixed overnight in the same fixative and were cryoprotected in 30% sucrose and cut using a cryostat. The sample was incubated with primary antibody dilution 1/3000 (PBS + 0.3% Triton X100) at 20°C for 18 hours. The Alexa Fluor® 488 conjugated Goat polyclonal to rabbit IgG dilution 1/1000 was used as secondary antibody. The image shows staining in the soma and processes of neurons with the expected distribution of Neuropeptide Y.
Anti-Neuropeptide Y antibody (ab30914)参考文献
This product has been referenced in:
- Raghanti MA et al. Humans and great apes share increased neocortical neuropeptide Y innervation compared to other haplorhine primates. Front Hum Neurosci 8:101 (2014). IHC ; Human . Read more (PubMed: 24616688) »
- Heinrich G et al. Preserved energy balance in mice lacking FoxO1 in neurons of Nkx2.1 lineage reveals functional heterogeneity of FoxO1 signaling within the hypothalamus. Diabetes 63:1572-82 (2014). Mouse . Read more (PubMed: 24487022) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"