Endogenous ligand for the NPS receptor involved in many biological functions including sleeping/wakening, locomotion, anxiety, and food intake.
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Soluble in Water
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Functional Studies - Neuropeptide S (rat) (ab120246)
ab70280 staining cAMP in HEK293 cells treated with neuropeptide S (ab120246), by ICC/IF. Increase in cAMP expression correlates with increased concentration of neuropeptide S, as described in literature. The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120246 (neuropeptide S) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab70280 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
This product has been referenced in:
Reinscheid RK Neuropeptide S: anatomy, pharmacology, genetics and physiological functions. Results Probl Cell Differ46:145-58 (2008).
Read more (PubMed: 18204825) »
Xu YL et al. Distribution of neuropeptide S receptor mRNA and neurochemical characteristics of neuropeptide S-expressing neurons in the rat brain. J Comp Neurol500:84-102 (2007).
Read more (PubMed: 17099900) »