Western blot - Neuronal membrane glycoprotein M6-a antibody (ab79128)
All lanes : Anti-Neuronal membrane glycoprotein M6-a antibody (ab79128) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466) Lane 2 : Human brain normal tissue lysate - membrane extract (ab29456) Lane 3 : Brain (Mouse) Tissue Lysate Lane 4 : Spinal Cord (Mouse) Tissue Lysate Lane 5 : Brain (Rat) Tissue Lysate Lane 6 : Spinal Cord (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 31 kDa Observed band size : 31 kDa Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.
ICC/IF image of ab79128 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab79128 at 10µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.