概述

  • 产品名称Neurokinin A
  • 描述
    Tachykinin肽
  • 别名
    • NKA
  • 生物学描述Endogenous tachykinin peptide
  • 纯度> 95%

性能

    应用

    Our Abpromise guarantee covers the use of ab120185 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    Neurokinin A 图像

    • K562 cells were incubated at 37°C for 4h with vehicle control (0 μM) and 15 nM of neurokinin A (ab120185). Increased expression of p53 (ab26) correlates with an increase in neurokinin A concentration, as described in literature.

      Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab26 at 5 μg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP (ab97040) at 1/10000 dilution and visualised using ECL development solution.

    Neurokinin A (ab120185)参考文献

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