Anti-Neurogranin抗体(ab99269)

概述

  • 产品名称Anti-Neurogranin抗体
    参阅全部 Neurogranin 一抗
  • 描述
    山羊多克隆抗体to Neurogranin
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Dog
  • 免疫原

    Synthetic peptide:

    C-KIKSGERGRKG

    , corresponding to internal sequence amino acids 45-55 of Human Neurogranin (NP_006167.1).

  • 阳性对照
    • Human brain cerebral cortex lysate, Human brain hippocampus lysate, Mouse brain lysate and Rat brain lysate.

性能

应用

Our Abpromise guarantee covers the use of ab99269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 8 kDa). An approximately 15 kDa band is observed in Human Brain (Cerebral Cortex and Hippocampus), and in Mouse Brain and Rat Brain lysates. There is also an additional 16kDa band. The observed molecular weights correspond to earlier findings in the literature with different antibodies.

靶标

  • 功能Acts as a "third messenger" substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium.
  • 组织特异性In the cerebral cortex, found in the cell bodies of neurons in layers II-VI, and in apical and basal dendrites of pyramidal neurons. Is not found in the dendrites in patients with Alzheimer disease.
  • 序列相似性Belongs to the neurogranin family.
    Contains 1 collagen-like domain.
    Contains 1 IQ domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Protein kinase C substrate RC3 antibody
    • Calmodulin binding protein antibody
    • Hng antibody
    • NEUG(55-78) antibody
    • NEUG_HUMAN antibody
    • Neurogranin (protein kinase C substrate) antibody
    • Ng antibody
    • NRGN antibody
    • Protein kinase C substrate antibody
    • RC3 antibody
    see all

Anti-Neurogranin antibody 图像

  • All lanes : Anti-Neurogranin antibody (ab99269) at 2 µg/ml

    Lane 1 : Human cerebellum lysates
    Lane 2 : Rat brain lysates

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 8 kDa
  • Anti-Neurogranin antibody (ab99269) at 0.1 µg/ml + Mouse brain lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 8 kDa
    Primary incubation was 1 hour.
  • ab99269 (3.8µg/ml) staining of paraffin embedded Human Cortex shows cytoplasm staining in the neuronal cell bodies. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-Neurogranin antibody (ab99269)参考文献

ab99269 has not yet been referenced specifically in any publications.

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