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Our Abpromise guarantee covers the use of ab23570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 21346800|
|IHC-Fr||1/500. Fix fresh frozen sections with PFA.|
|WB||1/1000 - 1/2000. Predicted molecular weight: 7 kDa.|
ab23570 at 1/500 staining mouse embryonic mesencephalon tissue sections by IHC-Fr. The sections were paraformaldehyde fixed and incubated with the antibody for 12 hours. An Alexa Fluor ® 488 conjugated goat polyclonal antibody was used as the secondary.