概述

  • 产品名称Anti-Neurogranin抗体
    参阅全部 Neurogranin 一抗
  • 描述
    兔多克隆抗体to Neurogranin
  • 经测试应用适用于: IHC-P, IHC-FoFr, IHC-Fr, WB, ICC, IP, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Cow, Human
  • 免疫原

    Recombinant full length protein (Rat).

性能

应用

Our Abpromise guarantee covers the use of ab23570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21346800
IHC-Fr 1/500. Fix fresh frozen sections with PFA.
WB 1/1000 - 1/2000. Predicted molecular weight: 7 kDa.
ICC 1/500.
IP 1/100.
ELISA 1/2000.

靶标

  • 功能Acts as a "third messenger" substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium.
  • 组织特异性In the cerebral cortex, found in the cell bodies of neurons in layers II-VI, and in apical and basal dendrites of pyramidal neurons. Is not found in the dendrites in patients with Alzheimer disease.
  • 序列相似性Belongs to the neurogranin family.
    Contains 1 collagen-like domain.
    Contains 1 IQ domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Protein kinase C substrate RC3 antibody
    • Calmodulin binding protein antibody
    • Hng antibody
    • NEUG(55-78) antibody
    • NEUG_HUMAN antibody
    • Neurogranin (protein kinase C substrate) antibody
    • Ng antibody
    • NRGN antibody
    • Protein kinase C substrate antibody
    • RC3 antibody
    see all

Anti-Neurogranin antibody 图像

  • ab23570 at 1/500 staining mouse embryonic mesencephalon tissue sections by IHC-Fr. The sections were paraformaldehyde fixed and incubated with the antibody for 12 hours. An Alexa Fluor ®  488 conjugated goat polyclonal antibody was used as the secondary.

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Anti-Neurogranin antibody (ab23570)参考文献

This product has been referenced in:

See all 3 Publications for this product

Product Wall

The recombinant protein immunogen for ab23570 did not contain any tag.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (cortical neurons)
Loading amount 30 µg
Specification cortical neurons
Gel Running Conditions Reduced Denaturing (4-12% SDS)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 4% · Temperature: 25°C
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提交于 Feb 08 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (embryonic mesencephalon)
Specification embryonic mesencephalon
Fixative Paraformaldehyde
Permeabilization No
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提交于 Aug 03 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"