Anti-Neuroglycan C抗体(ab31946)

概述

  • 产品名称Anti-Neuroglycan C抗体
    参阅全部 Neuroglycan C 一抗
  • 描述
    兔多克隆抗体to Neuroglycan C
  • 经测试应用适用于: ICC/IF, IHC-FoFr, WBmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Cow, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Mouse Neuroglycan C.

    (Peptide available as ab32755.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: Spinal Cord (Mouse) Tissue Lysate Spinal Cord (Rat) Lysate. Brain (Mouse) Tissue Lysate Brain (Rat) Tissue Lysate

性能

应用

Our Abpromise guarantee covers the use of ab31946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
IHC-FoFr
WB
  • 应用说明ICC/IF: Use at a concentration of 1 µg/ml.
    IHC-FoFr: 1/300 (See abreview for more details)
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 60,45 kDa (predicted molecular weight: 60,58,48 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能May function as a growth and differentiation factor involved in neuritogenesis. May induce ERBB3 activation.
    • 组织特异性Restricted to brain (at protein level).
    • 序列相似性Contains 1 EGF-like domain.
    • 发展阶段Expressed in brain of 3 months, 5 and 10-year-old individuals.
    • 翻译后修饰N-glycosylated.
      O-glycosylated; contains chondroitin sulfate glycans. Part-time proteoglycan, expressed in part as a proteoglycan exhibiting chondroitin sulfate glycans and in part as a non-proteoglycan form. The relative amount of both forms depends on tissues and tissues maturation.
      Phosphorylated; in intracellular and extracellular parts.
    • 细胞定位Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. In neurons, localizes to synaptic junctions (By similarity). Also detected in the endoplasmic reticulum and the Golgi (By similarity). Partially enriched in lipid rafts.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Acidic leucine rich EGF like domain containing brain protein antibody
      • Acidic leucine-rich EGF-like domain-containing brain protein antibody
      • Caleb antibody
      • Chondroitin sulfate proteoglycan 5 (neuroglycan C) antibody
      • Chondroitin sulfate proteoglycan 5 antibody
      • CSPG5 antibody
      • CSPG5_HUMAN antibody
      • MGC44034 antibody
      • Neuroglycan C antibody
      • Ngc antibody
      • OTTHUMP00000164796 antibody
      • OTTHUMP00000164797 antibody
      • OTTHUMP00000210429 antibody
      see all

    Anti-Neuroglycan C antibody 图像

    • All lanes : Anti-Neuroglycan C antibody (ab31946) at 1 µg/ml

      Lane 1 : Mouse Spinal Cord Lysate
      Lane 2 : Rat Spinal Cord Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

      Performed under reducing conditions.

      Predicted band size : 60,58,48 kDa
      Observed band size : 45,60 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-Neuroglycan C antibody (ab31946) at 1 µg/ml

      Lane 1 : Brain (Mouse) Tissue Lysate
      Lane 2 : Brain (Rat) Tissue Lysate - normal tissue

      Lysates/proteins at 10 µg per lane.

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 60,58,48 kDa
      Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    • ICC/IF image of ab31946 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31946, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
    • ab31946 staining Neuroglycan C in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/300 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG was used at 1/1000 dilution, as secondary.

      This image is courtesy of an Abreview submitted by Dr Sophie Pezet.

    Anti-Neuroglycan C antibody (ab31946)参考文献

    ab31946 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Rat Tissue sections (Brain sections)
    Specification Brain sections
    Fixative Paraformaldehyde
    Antigen retrieval step None
    Permeabilization No
    Username

    Dr. Sophie Pezet

    Verified customer

    提交于 Jun 05 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"