Anti-Neuroglobin抗体[13C8] (ab37258)

概述

  • 产品名称Anti-Neuroglobin抗体[13C8]
    参阅全部 Neuroglobin 一抗
  • 描述
    小鼠单克隆抗体[13C8] to Neuroglobin
  • 经测试应用适用于: WB, ELISA, Flow Cyt, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant full length protein (Human).

  • 阳性对照
    • Neuroglobin is mainly expressed in brain and retina.

性能

应用

Our Abpromise guarantee covers the use of ab37258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.
ELISA Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P 1/50 - 1/100.

靶标

  • 功能Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis.
  • 组织特异性Predominantly expressed in brain, the strongest expression is seen in the frontal lobe, the subthalamic nucleus and the thalamus.
  • 序列相似性Belongs to the globin family.
  • 翻译后修饰A redox disulfide bond regulates the heme pocket coordination and the rate of nitrite reduction to NO.
    Phosphorylated in vitro by ERK1, ERK2 and PKA, and in vivo during hypoxia. Phosphorylation increases nitrite reductase activity.
  • 细胞定位Perikaryon. Cytoplasm. Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Neuroglobin antibody
    • NGB antibody
    • NGB_HUMAN antibody

Anti-Neuroglobin antibody [13C8] 图像

  • Overlay histogram showing SH-SY5Y cells stained with ab37258 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab37258, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
  • Anti-Neuroglobin antibody [13C8] (ab37258) at 1 µg/ml + Recombinant Human Neuroglobin protein (ab63278) at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 4 minutes

Anti-Neuroglobin antibody [13C8] (ab37258)参考文献

This product has been referenced in:
  • Ord EN  et al. Combined antiapoptotic and antioxidant approach to acute neuroprotection for stroke in hypertensive rats. J Cereb Blood Flow Metab 33:1215-24 (2013). Rat . Read more (PubMed: 23632970) »
  • Raychaudhuri S  et al. Neuroglobin protects nerve cells from apoptosis by inhibiting the intrinsic pathway of cell death. Apoptosis 15:401-11 (2010). WB ; Human . Read more (PubMed: 20091232) »

See all 5 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (brain)
Permeabilization Yes - Phosphate Buffer (pH 7.2)+ 0,03% Triton X
Specification brain
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 16 2015

Application Western blot
Sample Rat Tissue lysate - whole (Hippocampus, cortex)
Loading amount 150 µg
Specification Hippocampus, cortex
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Saurav Bhowmick

Verified customer

提交于 Aug 03 2012

Thank you very much for your interest in Abcam anti-neuroglobin antibodies.

My Spanish speaking colleague is currently out of the office and unfortunately my Spanish is not good enough to reply to you in your language. I hope this is fine with...

Read More

Thank you for your reply and I do apologize for the confusion.

The discount code information is already in your Abreview, so please just click on the link below and then re-send your Abreview back to us. Once you have re-sent the Abreview to u...

Read More

Thank you for your reply.

I actually just noticed that the discount code is already in the 'Additional Notes' section, sorry I missed that previoulsy.

Therefore, you are all set, and so you can go ahead and use the discount code.
...

Read More

Thank you for contacting Abcam.

I have just returned you Abreview to you, so that you can input the testing discount code into the Abreview. Once you have resubmitted that Abreview, with the testing discount code information, you can go ahead ...

Read More

DISCOUNT CODE: *
Expiration date: *

I am very pleased to hear you would like to accept our offer and test ab37258 in rat tissue. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please subm...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain, Cortex)
Specification Brain, Cortex
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Dr. Dominik Schelshorn

Verified customer

提交于 Feb 04 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"