Anti-Neurogenin 1抗体(ab89461)

概述

  • 产品名称Anti-Neurogenin 1抗体
    参阅全部 Neurogenin 1 一抗
  • 描述
    小鼠单克隆抗体to Neurogenin 1
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Chicken
  • 免疫原

    Recombinant fragment: SEALLHSLRR SRRVKANDRE RNRMHNLNAA LDALRSVLPS FPDDTKLTKI ETLRFAYNYI WALAETLRLA DQGLPGGGAR ER, corresponding to amino acids 82-163 of human Neurogenin 1 (NP_006152) with a proprietary tag.

  • 阳性对照
    • WB: The immunoge; HeLa nuclear extract; Y-79 cell lysate ICC/IF: HeLa cell

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab89461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.
ELISA Use at an assay dependent dilution. Detection limit for recombinant, tagged Neurogenin 1 is approximately 10 ng/ml when used as a capture antibody.
ICC/IF Use a concentration of 50 µg/ml.

靶标

Anti-Neurogenin 1 antibody 图像

  • Anti-Neurogenin 1 antibody (ab89461) at 1 µg/ml + recombinant, tagged human Neurogenin 1 protein fragment (the immunogen) at 0.2 µg

    Predicted band size : 26 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • Anti-Neurogenin 1 antibody (ab89461) at 1 µg/ml + HeLa nuclear extract at 50 µg

    Predicted band size : 26 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
  • Anti-Neurogenin 1 antibody (ab89461) at 1 µg/ml + Y-79 cell lysate at 50 µg

    Predicted band size : 26 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
  • ab89461, at 50 µg/ml, staining Neurogenin 1 in HeLa cell by Immunofluorescence.

Anti-Neurogenin 1 antibody (ab89461)参考文献

This product has been referenced in:
  • Jahan I  et al. Expression of neurog1 instead of atoh1 can partially rescue organ of corti cell survival. PLoS One 7:e30853 (2012). WB ; Mouse . Read more (PubMed: 22292060) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (E11.5 mouse embryos transverse section)
Specification E11.5 mouse embryos transverse section
Fixative Paraformaldehyde
Permeabilization Yes - PBST (PBS / 0.5% v/v Triton X-100 (Sigma))
Blocking step NOVOCASTRA protein block as blocking agent as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 13 2013

Application Western blot
Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount 50 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Jan 28 2013

DISCOUNT CODES: 100%ABR-E7TFV for testing ab89461 in Zebrafish 100%ABR-E7TF3 for testing ab67168 in Zebrafish 100%ABR-E7TFM for testing ab101699 in Zebrafish Expiration date for all the three codes: 22. February 2012 I am very pleased to hear you would...

Read More

Thank you very much for your inquiry. Indeed, it is difficult to find antibodies crossreactive with Zebrafish. I don't know whether I have already told you about our list of Zebrafish crossreactive antibodies? It can be found under the following link: ...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Apteronotus leptorhynchus Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton X-100
Blocking step 3% sheep serum, 1% BSA, 1% teleostean gelatine in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Username

Dr. Ruxandra Sirbulescu

Verified customer

提交于 Oct 05 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (SVZ Cell Culture)
Loading amount 40 µg
Specification SVZ Cell Culture
Gel Running Conditions Reduced Denaturing (12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Jun 22 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"