Anti-Neurogenin 1抗体(ab66498)

概述

  • 产品名称Anti-Neurogenin 1抗体
    参阅全部 Neurogenin 1 一抗
  • 描述
    兔多克隆抗体to Neurogenin 1
  • 经测试应用适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from the C terminal domain of human Neurogenin 1.

性能

应用

Our Abpromise guarantee covers the use of ab66498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1000.
ICC/IF 1/1000.
WB 1/500 - 1/5000. Predicted molecular weight: 26 kDa.

靶标

  • 功能Appears to mediate neuronal differentiation.
  • 组织特异性Expression restricted to the embryonic nervous system.
  • 序列相似性Contains 1 basic helix-loop-helix (bHLH) domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AKA antibody
    • Ath4c antibody
    • bHLHa6 antibody
    • Class A basic helix-loop-helix protein 6 antibody
    • Helix loop helix protein mATH 4C antibody
    • MATH4C antibody
    • NeuroD3 antibody
    • Neurog 1 antibody
    • Neurog1 antibody
    • Neurogenic basic helix loop helix protein antibody
    • Neurogenic basic-helix-loop-helix protein antibody
    • Neurogenic differentiation 3 antibody
    • Neurogenic differentiation factor 3 antibody
    • Neurogenin-1 antibody
    • Neurogenin1 antibody
    • Ngn antibody
    • NGN-1 antibody
    • Ngn1 antibody
    • NGN1_HUMAN antibody
    see all

Anti-Neurogenin 1 antibody 图像

  • ICC/IF image of ab66498 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66498, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Ab66498 staining human normal liver. Staining is localised to the nucleus.
    Left panel: with primary antibody diluted 1:1000. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Neurogenin 1 antibody (ab66498)参考文献

This product has been referenced in:
  • Blanchard JW  et al. Selective conversion of fibroblasts into peripheral sensory neurons. Nat Neurosci 18:25-35 (2015). IHC (PFA fixed) ; Mouse . Read more (PubMed: 25420069) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (E11.5 mouse embryos transverse section)
Specification E11.5 mouse embryos transverse section
Fixative Paraformaldehyde
Permeabilization Yes - PBST (PBS / 0.5% v/v Triton X-100 (Sigma))
Blocking step NOVOCASTRA protein block as blocking agent as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 May 13 2013

Thank you for your call today and for letting us know about the trouble with ab66498. As we discussed, I am sending a free of charge vial of ab89461 on the order ***, which I expect to arrive Friday of next week or shortly after. Please ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"