Anti-Neurofilament heavy polypeptide抗体[RNF404] (ab8972)
Key features and details
- Mouse monoclonal [RNF404] to Neurofilament heavy polypeptide
- Suitable for: IHC-P
- Reacts with: Rat, Human
- Isotype: IgG2a
概述
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产品名称
Anti-Neurofilament heavy polypeptide抗体[RNF404]
参阅全部 Neurofilament heavy polypeptide 一抗 -
描述
小鼠单克隆抗体[RNF404] to Neurofilament heavy polypeptide -
宿主
Mouse -
特异性
Reacts with the intermediate filament protein of periferal and central nerve cells, corresponding in Western blotting with the 200 kDa protein. Specific for the phosphorylated isoform. -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Rat, Human -
免疫原
Tissue, cells or virus corresponding to Cow Neurofilament heavy polypeptide. Neurofilament preparation of calf brain tissue.
Database link: P12036 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
RNF404 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgG2a -
研究领域
相关产品
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Affibody® Molecule
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/50 - 1/100.
|
说明 |
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IHC-P
1/50 - 1/100. |
靶标
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功能
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. -
疾病相关
Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors. -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization. - Information by UniProt
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数据库链接
- Entrez Gene: 4744 Human
- Entrez Gene: 24587 Rat
- Omim: 162230 Human
- SwissProt: P12036 Human
- SwissProt: P16884 Rat
- Unigene: 198760 Human
- Unigene: 108194 Rat
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别名
- 200 kDa neurofilament protein antibody
- CMT2CC antibody
- Nefh antibody
see all
图片
数据表及文件
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Datasheet download
文献 (1)
ab8972 被引用在 1 文献中.
- Petruska JM et al. A Transient Developmental Background Finding in the Retina Observed in Neonatal Dogs in Juvenile Toxicology Studies. Toxicol Pathol 47:528-541 (2019). PubMed: 31064296