Anti-Neurofilament heavy polypeptide抗体[RNF402] (ab3966)


  • 产品名称Anti-Neurofilament heavy polypeptide抗体[RNF402]
    参阅全部 Neurofilament heavy polypeptide 一抗
  • 描述
    小鼠单克隆抗体[RNF402] to Neurofilament heavy polypeptide
  • 特异性Axonal Marker. This antibody reacts with the intermediate filament protein of central and periferal nerve cells. In Western blotting this antibody is reactive with the Neurofilament heavy polypeptide protein only. This antibody reacts with both the phosphorylated and non-phosphorylated isoforms.
  • 经测试应用适用于: IHC-P, IHC-Fr, WB, ICCmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Xenopus laevis, Monkey
  • 免疫原

    Neurofilament preparation of calf brain tissue.



Our Abpromise guarantee covers the use of ab3966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/10 - 1/100.
IHC-Fr 1/10 - 1/100.
WB 1/25 - 1/250.
ICC Use at an assay dependent concentration.

Permeabilised cells


  • 功能Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
  • 疾病相关Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
  • 序列相似性Belongs to the intermediate filament family.
  • 翻译后修饰There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
    Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 200 kDa neurofilament protein antibody
    • CMT2CC antibody
    • Nefh antibody
    • Neurofilament heavy polypeptide 200kDa antibody
    • Neurofilament heavy polypeptide antibody
    • Neurofilament triplet H protein antibody
    • NF H antibody
    • NF-H antibody
    • NFH antibody
    • NFH_HUMAN antibody
    see all

Anti-Neurofilament heavy polypeptide antibody [RNF402] 图像

  • Immunohistochemistry on frozen section of rat brain

Anti-Neurofilament heavy polypeptide antibody [RNF402] (ab3966)参考文献

This product has been referenced in:
  • Han S  et al. The collagen scaffold with collagen binding BDNF enhances functional recovery by facilitating peripheral nerve infiltrating and ingrowth in canine complete spinal cord transection. Spinal Cord 52:867-73 (2014). Read more (PubMed: 25311846) »
  • Haro H  et al. Experimental chemonucleolysis with recombinant human matrix metalloproteinase 7 in human herniated discs and dogs. Spine J N/A:N/A (2013). Human . Read more (PubMed: 24295797) »

See all 2 Publications for this product

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Thank you for your enquiry. This antibody is IgM kappa, we don't have any additional information. If you have any additional questions, please contact us again.