Anti-Neurofilament heavy polypeptide抗体[NF-05] (ab118812)

概述

  • 产品名称Anti-Neurofilament heavy polypeptide抗体[NF-05]
    参阅全部 Neurofilament heavy polypeptide 一抗
  • 描述
    小鼠单克隆抗体[NF-05] to Neurofilament heavy polypeptide
  • 经测试应用适用于: WB, ELISA, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Pig
  • 免疫原

    Full length native protein (purified) corresponding to Human Neurofilament heavy polypeptide. This antibody was raised against pig brain neurofilament protein-enriched fraction after depolymerization of microtubules.
    Database link: P12036

  • 阳性对照
    • Human Brain (Cerebellum and Cortex) and Peripheral Nerve tissues; Human brain homogenate lysate.

性能

应用

Our Abpromise guarantee covers the use of ab118812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 113 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
  • 疾病相关Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
  • 序列相似性Belongs to the intermediate filament family.
  • 翻译后修饰There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
    Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 200 kDa neurofilament protein antibody
    • CMT2CC antibody
    • Nefh antibody
    • Neurofilament heavy polypeptide 200kDa antibody
    • Neurofilament heavy polypeptide antibody
    • Neurofilament triplet H protein antibody
    • NF H antibody
    • NF-H antibody
    • NFH antibody
    • NFH_HUMAN antibody
    see all

Anti-Neurofilament heavy polypeptide antibody [NF-05] 图像

  • ab118812, at 10 µg/ml, staining Neurofilament heavy polypeptide in formalin-fixed, paraffin-embedded Human Brain (Cerebellum) tissue by Immunohistochemistry.

  • ab118812, at 10 µg/ml, staining Neurofilament heavy polypeptide in formalin-fixed, paraffin-embedded Human Brain (Cortex) tissue by Immunohistochemistry.

  • ab118812, at 10 µg/ml, staining Neurofilament heavy polypeptide in formalin-fixed, paraffin-embedded Human Peripheral Nerve tissue by Immunohistochemistry.

Anti-Neurofilament heavy polypeptide antibody [NF-05] (ab118812)参考文献

ab118812 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your email.
The size difference will effect WB not IHC. However it will more depend on the tissues section you will be using.

Please check the localization of different chains in axons
http://www.uniprot.org/uniprot/P...

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Thank you for contacting us. I am afraid we do not have exact match of monoclonal the customer is looking for. We however have many antibodies that are against the neurofilament proteins that forms part of Axons. As by definition Neurofilaments (NFs) a...

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