Anti-Neurofilament heavy polypeptide抗体(ab8135)

概述

  • 产品名称
    Anti-Neurofilament heavy polypeptide抗体
    参阅全部 Neurofilament heavy polypeptide 一抗
  • 描述
    兔多克隆抗体to Neurofilament heavy polypeptide
  • 特异性
    The antibody recognizes both phosphorylated and non-phosphorylated forms of NF-H. Specifically recognizes the heavy microfilament subunit (~180-220 kDa).
  • 经测试应用
    适用于: IHC-FoFr, ICC/IF, ICC, IHC-P, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Cat, Human, Pig
  • 免疫原

    Recombinant fragment corresponding to Cow Neurofilament heavy polypeptide (C terminal).

性能

应用

Our Abpromise guarantee covers the use of ab8135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration. PubMed: 19075249
ICC/IF 1/1000.
ICC Use at an assay dependent concentration.
IHC-P 1/1000.
IHC-Fr 1/1000.
WB 1/10000.

靶标

  • 功能
    Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
  • 疾病相关
    Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
  • 序列相似性
    Belongs to the intermediate filament family.
  • 翻译后修饰
    There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
    Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 200 kDa neurofilament protein antibody
    • CMT2CC antibody
    • Nefh antibody
    • Neurofilament heavy polypeptide 200kDa antibody
    • Neurofilament heavy polypeptide antibody
    • Neurofilament triplet H protein antibody
    • NF H antibody
    • NF-H antibody
    • NFH antibody
    • NFH_HUMAN antibody
    see all

Anti-Neurofilament heavy polypeptide antibody 图像

  • ab8135 staining Neurofilament heavy polypeptide in rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections).
    Tissue was fixed with formaldehyde, blocked with 5% serum for 1 hour at 25°C and permeabilzed with 0.1% Triton X-100. Samples were incubated with primary antibody (1/500 in diluent) for 12 hours at 25°C. An Alexa Fluor® 555 goat anti-rabbit polyclonal IgG (1/300) was used as the secondary antibody.

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  • Immunocytochemical staining of 33B cells (rat oligodendroglioma) with ab8135 at 1/1000. Cells were cultured in DMEM/10% FCS on coverslips in 12 well plates. Cells were washed with PBS and fixed in 4% PFA until staining. Cells were washed teice for 2 minutes and then treated with 3% hydrogen peroxide in methanol for 15 minutes at room temperature. The sample was blocked in 10% goat serum in 1% PBSA and then incubated with the primary antibody in 1% PBSA for 2 hours at room temperature. A biotinylated goat anti-rabbit was used as the secondary at 1/200 and then incubated with ABC for 30 minutes at room temperature, followed by DAB in PBS (brown). The sample was counter-stained with haematoxylin.

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  • The image showing results obtained after protease pretreatment, illustrating high definition neurofilament staining. This picture was kindly supplied as part of the review submitted by Prof Colm Cunningham and Dr Suzanne Campion (Southampton University).
  • ab8135 at 1/500 staining aged muscle tissue sections from Rat by IHC-Fr.

    The tissue section was permeabilized with Triton X100 and blocked with 10% serum for 30 minutes at 25°C.

    The primary antibody was incubated for 1 hour at 25°C.

    Muscle was stained without fixation and post-fixed in 10% NBF following staining for 1h.

    Green = alpha bungarotoxin stained post-synaptic neuromuscular junction.

    Red = ab8135 staining Neurofilament heavy polypeptide

     

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Anti-Neurofilament heavy polypeptide antibody (ab8135)参考文献

This product has been referenced in:
  • Vasconcelos DM  et al. Immune response and innervation signatures in aseptic hip implant loosening. J Transl Med 14:205 (2016). Read more (PubMed: 27387445) »
  • Gao Y  et al. Neuroprotective effects of polydatin against mitochondrial-dependent apoptosis in the rat cerebral cortex following ischemia/reperfusion injury. Mol Med Rep 14:5481-5488 (2016). ICC/IF ; Rat . Read more (PubMed: 27840959) »

See all 17 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% Normal buffered formalin
Username

Abcam user community

Verified customer

提交于 Dec 05 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% Normal buffered formalin
Username

Abcam user community

Verified customer

提交于 Dec 05 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cultured Cells (engineered nervous tissue)
Permeabilization
No
Specification
engineered nervous tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 22 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric buffer, pH6.0
Sample
Human Tissue sections (fetal spinal cord)
Specification
fetal spinal cord
Permeabilization
Yes - Tween-20
Fixative
Paraformaldehyde
Username

Mr. Heiko Locher

Verified customer

提交于 Sep 23 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cell (Kelly Neuroblastoma Cell line)
Specification
Kelly Neuroblastoma Cell line
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Sep 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Neuro2a)
Loading amount
6 µg
Specification
Neuro2a
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Abcam user community

Verified customer

提交于 Aug 22 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
None
Permeabilization
Yes - 0.1% TritonX-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 16 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (B50 Cell line)
Loading amount
6 µg
Specification
B50 Cell line
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Jul 12 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Rat Cultured Cells (33B)
Specification
33B
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Jul 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Neuro2a Cell Line)
Specification
Neuro2a Cell Line
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Jul 06 2012

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