Anti-Neurofilament heavy polypeptide抗体(ab72996)


  • 产品名称Anti-Neurofilament heavy polypeptide抗体
    参阅全部 Neurofilament heavy polypeptide 一抗
  • 描述
    鸡多克隆抗体to Neurofilament heavy polypeptide
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Cow, Human
  • 免疫原

    Full length native protein (purified) corresponding to Cow Neurofilament heavy polypeptide.

  • 阳性对照
    • Rat cerebral cortex lysate. Rat cortical neurons and glia.



Our Abpromise guarantee covers the use of ab72996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50000. Detects a band of approximately 200 kDa (predicted molecular weight: 115 kDa).
ICC/IF 1/25000.


  • 功能Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
  • 疾病相关Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
  • 序列相似性Belongs to the intermediate filament family.
  • 翻译后修饰There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
    Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 200 kDa neurofilament protein antibody
    • CMT2CC antibody
    • Nefh antibody
    • Neurofilament heavy polypeptide 200kDa antibody
    • Neurofilament heavy polypeptide antibody
    • Neurofilament triplet H protein antibody
    • NF H antibody
    • NF-H antibody
    • NFH antibody
    • NFH_HUMAN antibody
    see all

Anti-Neurofilament heavy polypeptide antibody 图像

  • Anti-Neurofilament heavy polypeptide antibody (ab72996) at 1/50000 dilution + Rat cerebral cortex lysate

    Predicted band size : 115 kDa
    Observed band size : 200 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence of rat cortical neurons and glia using ab72996 at 1/25000 dilution showing Neurofilament heavy polypeptide staining (red).

Anti-Neurofilament heavy polypeptide antibody (ab72996)参考文献

This product has been referenced in:
  • Schrenk-Siemens K  et al. PIEZO2 is required for mechanotransduction in human stem cell-derived touch receptors. Nat Neurosci 18:10-6 (2015). Read more (PubMed: 25469543) »
  • Lucas EK  et al. PGC-1a provides a transcriptional framework for synchronous neurotransmitter release from parvalbumin-positive interneurons. J Neurosci 34:14375-87 (2014). WB ; Mouse . Read more (PubMed: 25339750) »

See all 3 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 7% · Temperature: 25°C
Sample Mouse Cell (DRG neurons)
Specification DRG neurons
Permeabilization Yes - 0.1% triton
Fixative Paraformaldehyde

Mr. Shachar Dagan

Verified customer

提交于 Dec 16 2013