Anti-Neurofilament heavy polypeptide抗体(ab4680)

概述

  • 产品名称
    Anti-Neurofilament heavy polypeptide抗体
    参阅全部 Neurofilament heavy polypeptide 一抗
  • 描述
    鸡多克隆抗体to Neurofilament heavy polypeptide
  • 经测试应用
    适用于: IHC-Fr, IHC-FoFr, IHC - Wholemount, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Cat, Human, Aplysia
  • 免疫原

    Full length native protein (cow intermediate filaments were prepared from spinal cords by the glycerol polymerization method of Delacourte et al., and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE-cellulose and the NF-H containing fractions were concentrated and further purified by preparative gel electrophoresis on a Biorad Prepcell).

性能

应用

Our Abpromise guarantee covers the use of ab4680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/10000.
IHC-FoFr 1/1000.
IHC - Wholemount Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 19944698
WB 1/1e+006. Predicted molecular weight: 200-220 kDa.

靶标

  • 功能
    Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
  • 疾病相关
    Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
  • 序列相似性
    Belongs to the intermediate filament family.
  • 翻译后修饰
    There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
    Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 200 kDa neurofilament protein antibody
    • CMT2CC antibody
    • Nefh antibody
    • Neurofilament heavy polypeptide 200kDa antibody
    • Neurofilament heavy polypeptide antibody
    • Neurofilament triplet H protein antibody
    • NF H antibody
    • NF-H antibody
    • NFH antibody
    • NFH_HUMAN antibody
    see all

Anti-Neurofilament heavy polypeptide antibody 图像

  • Immunohistochemical staining of Neurofilament heavy polypeptide in aplysia buccal ganglia with ab4680 at 1/1000. The sample was permeabilized with 2% Triton X100 and blocked with 1%BSA/5% goat serum for 2 hours at room temperature, before being incubated with the primary antibody for 1 hour at room temperature in 5% goat serum. ab96949 (goat anti-chicken IgY H&L DyLight® 594) was used as the secondary antibody at 1/200.

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  • Rat Neurons stained with NFH (red) and rabbit polyclonal to GFAP(green). The NF-H antibody was used at a dilution of 1/100000 and the GFAP at a dilution of 1:2,000 using our standard fixation and staining procedure (see protocol section).
  • Human neuroblastoma SH-SY5Y cells stained with chicken anti-NF-H (red) and mouse monoclonal to fibrillarin 38F3 (green). Nuclear DNA is revealed with Hoechst dye (blue). The NF-H antibody was used at a dilution of 1/100000 and the fibrillarin monoclonal at 1/1000. Cultures were processed using our standard fixation and staining procedure (in protocol section).
  • Rat Neurons stained with chicken anti-NF-H (green). The NF-H antibody was used at a dilution of 1/100000 using our standard fixation and staining procedure (in protocol section). 

  • ab4680 at 1/10000 dilution staining Neurofilament heavy polypeptide in rat skin tissue by immunohistochemistry (frozen sections). Sections were fixed using Bouin's Fixative and permeabilized in 0.1% Triton X-100 prior to blocking in 10% serum for 1 hour at 24°C and then incubated with ab4680 for 24 hours at 4°C. A donkey polyclonal to chicken IgY, diluted 1/100 was used as the secondary antibody.

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  • ab4680 staining 200 kD Neurofilament Heavy (green) in murine embryoid bodies by Immunocytochemistry/ Immunofluorescence. Nuclei were visualized with DAPI.

Anti-Neurofilament heavy polypeptide antibody (ab4680)参考文献

This product has been referenced in:
  • Esanov R  et al. The FMR1 promoter is selectively hydroxymethylated in primary neurons of fragile X syndrome patients. Hum Mol Genet 25:4870-4880 (2016). ICC/IF ; Human . Read more (PubMed: 28173181) »
  • Cabral CM  et al. Neurons are the Primary Target Cell for the Brain-Tropic Intracellular Parasite Toxoplasma gondii. PLoS Pathog 12:e1005447 (2016). IHC-Fr ; Mouse . Read more (PubMed: 26895155) »

See all 14 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (Differentiating Neuronal cells)
Permeabilization
Yes - Triton x-100, 0.01%
Specification
Differentiating Neuronal cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Fixative
Formaldehyde
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Abcam user community

Verified customer

提交于 Feb 17 2016

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 37°C
Sample
Pig Cell (Dorsal root ganglion neuron)
Specification
Dorsal root ganglion neuron
Permeabilization
Yes - Triton X
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 20 2015

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (dorsal root ganglian)
Specification
dorsal root ganglian
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

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Verified customer

提交于 Mar 16 2015

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
mix BSA 1% and Goat serum 5% as blocking agent for 2 hour(s) and 0 minute(s) · Temperature: 20°C
Antigen retrieval step
None
Sample
Rat Tissue sections (BRAIN)
Specification
BRAIN
Permeabilization
Yes - Triton 2%
Fixative
Paraformaldehyde
Username

Mrs. Francoise Geffroy

Verified customer

提交于 Apr 30 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA1% + Goat serum 5% as blocking agent for 2 hour(s) and 0 minute(s) · Temperature: 20°C
Antigen retrieval step
None
Sample
Aplysia Tissue sections (buccal ganglia)
Specification
buccal ganglia
Permeabilization
Yes - triton 2%
Fixative
Paraformaldehyde
Username

Mrs. Francoise Geffroy

Verified customer

提交于 Sep 30 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Mouse Tissue (never)
Specification
never
Username

Dr. Xinpeng Dun

Verified customer

提交于 Jul 05 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (scatic nerve)
Specification
scatic nerve
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.25% TX-100 30min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Username

Abcam user community

Verified customer

提交于 May 29 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Skin)
Specification
Skin
Fixative
Bouin's Fixative
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Aug 21 2009

Thank you for your enquiry. A positive control for immunocytochemistry would be mixed neuron/glial culture or frozen sections of brain or spinal cord. In either case rat, mouse or any other mammal should be fine. The antibody stains axons very stro...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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