Anti-Neurofilament heavy polypeptide抗体[3G3] (ab19386)


  • 产品名称Anti-Neurofilament heavy polypeptide抗体[3G3]
    参阅全部 Neurofilament heavy polypeptide 一抗
  • 描述
    小鼠单克隆抗体[3G3] to Neurofilament heavy polypeptide
  • 经测试应用适用于: IP, IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Neurofilament heavy polypeptide aa 1-226 (C terminal).

  • 阳性对照
    • Rat brain microsome



Our Abpromise guarantee covers the use of ab19386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 112 kDa).


  • 功能Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
  • 疾病相关Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
  • 序列相似性Belongs to the intermediate filament family.
  • 翻译后修饰There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
    Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 200 kDa neurofilament protein antibody
    • CMT2CC antibody
    • Nefh antibody
    • Neurofilament heavy polypeptide 200kDa antibody
    • Neurofilament heavy polypeptide antibody
    • Neurofilament triplet H protein antibody
    • NF H antibody
    • NF-H antibody
    • NFH antibody
    • NFH_HUMAN antibody
    see all

Anti-Neurofilament heavy polypeptide antibody [3G3] 图像

  • Immunofluorescence of neurofilament, heavy chain in rat cerebral cortex cultures in green.

Anti-Neurofilament heavy polypeptide antibody [3G3] (ab19386)参考文献

ab19386 has not yet been referenced specifically in any publications.

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