• 产品名称Anti-Neurofibromin抗体
    参阅全部 Neurofibromin 一抗
  • 描述
    兔多克隆抗体to Neurofibromin
  • 特异性ab30325 recognises neurofibromin.
  • 经测试应用适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human, Zebrafish
    预测可用于: Mouse, Rat, Pufferfish (Fugu), all Mammals
  • 免疫原

    Synthetic peptide:


    , corresponding to amino acids 27-41 of Human Neurofibromin



Our Abpromise guarantee covers the use of ab30325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/500.
WB 1/250 - 1/1000. Predicted molecular weight: 319 kDa.


  • 功能Stimulates the GTPase activity of Ras. NF1 shows greater affinity for Ras GAP, but lower specific activity. May be a regulator of Ras activity.
  • 疾病相关Defects in NF1 are the cause of neurofibromatosis type 1 (NF1) [MIM:162200]; also known as von Recklinghausen syndrome. A disease characterized by patches of skin pigmentation (cafe-au-lait spots), Lisch nodules of the iris, tumors in the peripheral nervous system and fibromatous skin tumors. Individuals with the disorder have increased susceptibility to the development of benign and malignant tumors.
    Defects in NF1 are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. Germline mutations of NF1 account for the association of JMML with type 1 neurofibromatosis (NF1).
    Defects in NF1 are the cause of Watson syndrome (WS) [MIM:193520]. WS is characterized by the presence of pulmonary stenosis, cafe-au-lait spots, and mental retardation. WS is considered as an atypical form of NF1.
    Defects in NF1 are a cause of familial spinal neurofibromatosis (FSNF) [MIM:162210]. Familial spinal NF is considered to be an alternative form of neurofibromatosis, showing multiple spinal tumors.
    Defects in NF1 are a cause of neurofibromatosis-Noonan syndrome (NFNS) [MIM:601321]. NFNS is characterized by manifestations of both NF1 and Noonan syndrome (NS). NS is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis.
    Defects in NF1 may be a cause of colorectal cancer (CRC) [MIM:114500].
  • 序列相似性Contains 1 CRAL-TRIO domain.
    Contains 1 Ras-GAP domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp686J1293 antibody
    • FLJ21220 antibody
    • Neurofibromatosis Noonan syndrome antibody
    • Neurofibromatosis related protein NF 1 antibody
    • Neurofibromatosis related protein NF1 antibody
    • neurofibromatosis type I antibody
    • Neurofibromatosis-related protein NF-1 antibody
    • Neurofibromin 1 antibody
    • Neurofibromin truncated antibody
    • Neurofibromin1 antibody
    • NF 1 antibody
    • NF antibody
    • NF1 antibody
    • NF1_HUMAN antibody
    • NFNS antibody
    • Type 1 Neurofibromatosis antibody
    • von Recklinghausen disease neurofibromin antibody
    • von Recklinghausen disease related protein VRNF antibody
    • VRNF antibody
    • WATS antibody
    • Watson disease related protein WSS antibody
    • Watson syndrome antibody
    • WSS antibody
    see all

Anti-Neurofibromin antibody 图像

  • ab30325 at a 1/500 dilution, staining human Neurofibromin in human dorsal ganglion neurons by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.

Anti-Neurofibromin antibody (ab30325)参考文献

This product has been referenced in:
  • Maertens O  et al. Elucidating distinct roles for NF1 in melanomagenesis. Cancer Discov 3:338-49 (2013). Human . Read more (PubMed: 23171796) »
  • Marrero D  et al. The neurofibromin 1 type I isoform predominance characterises female population affected by sporadic breast cancer: preliminary data. J Clin Pathol 65:419-23 (2012). IHC-P ; Human . Read more (PubMed: 22412049) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step hydrogen peroxide as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH6, 100C, 20min
Sample Human Tissue sections (placenta)
Specification placenta
Permeabilization No
Fixative Formaldehyde

Abcam user community

Verified customer

提交于 Jul 17 2014

Thank you for your reply.

I can confirm this antibody has been tested by another customer in IHC-P in an Abreview published on the datasheet. They tested in breast cancer tissue.The publications provided on the datasheet indicate they have u...

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Thank you for your message. I am very sorry to hear this antibody did not work well in mouse. It is regrettable this did not work for you.

We will be pleased to receive your results via an Abreview, as stated in the details regarding this of...

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Thank you for contacting us this afternoon.

As discussed over the phone, the anti-Neurofibromin antibody (ab30325) has not as yet been used in detecting the mouse protein as you are hoping to do. This does not mean that it would not work, sim...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Breast - Ca)
Specification Breast - Ca
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer (pH 9.0)
Permeabilization Yes - DAKO Wash-Buffer with Tween

Mr. Rudolf Jung

Verified customer

提交于 May 21 2012

Thank you for your question.

I am sorry to confirm that regrettablythe lab that provides this antibody considers the immunogenic peptide information to be proprietary in this case. The only information that we have is that the peptide is fro...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Zebrafish Tissue lysate - whole (3 day post fertilization zebrafish whole embryo ly)
Loading amount 50 µg
Specification 3 day post fertilization zebrafish whole embryo ly
Gel Running Conditions Reduced Denaturing (3-8% TA)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

提交于 Dec 09 2009