RabMAb

Anti-NeuroD2抗体[EPR5135] - ChIP Grade (ab109406)

概述

  • 产品名称Anti-NeuroD2抗体[EPR5135] - ChIP Grade
    参阅全部 NeuroD2 一抗
  • 描述
    兔单克隆抗体[EPR5135] to NeuroD2 - ChIP Grade
  • 经测试应用适用于: ChIP, WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC/IF or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human NeuroD2

  • 阳性对照
    • Human cerebellum, Human hippocampus, and fetal brain lysates; Human brain tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab109406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 41 kDa).
IHC-P 1/250 - 1/500. antigen retrieval is recommended.
  • 应用说明Is unsuitable for Flow Cyt,ICC/IF or IP.
  • 靶标

    Anti-NeuroD2 antibody [EPR5135] - ChIP Grade 图像

    • All lanes : Anti-NeuroD2 antibody [EPR5135] - ChIP Grade (ab109406) at 1/1000 dilution

      Lane 1 : Human cerebellum lysate
      Lane 2 : Human hippocampus lysate
      Lane 3 : Human fetal brain lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 41 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded Human brain tissue using 1/250 ab109406
    • ChIP analysis using ab109406 binding NeuroD2 in cerebral cortex tissue lysate from postnatal day 0 mouse. Cells were cross-linked for 10 minutes with 1% formaldehyde. Samples were incubated with primary antibody (1/200) for 2 hours at 4°C.

      Positive control: Known target of NeuroD2, which is the promoter regions of Nhlh2 gene.
      Primer sequence for PCR was:
      Nhlh2_F CTCACGAACTTCACCCGCAC
      Nhlh2_R AAATTGACTCCTCGGCCCTC
      Negative Control: ChIP performed with unrelated antibody (anti-GFP antibody).

      See Abreview

    Anti-NeuroD2 antibody [EPR5135] - ChIP Grade (ab109406)参考文献

    This product has been referenced in:
    • Bayam E  et al. Genome-wide target analysis of NEUROD2 provides new insights into regulation of cortical projection neuron migration and differentiation. BMC Genomics 16:681 (2015). Mouse . Read more (PubMed: 26341353) »
    • Katsushima K  et al. Contribution of MicroRNA-1275 to Claudin11 Protein Suppression via a Polycomb-mediated Silencing Mechanism in Human Glioma Stem-like Cells. J Biol Chem 287:27396-406 (2012). WB ; Human . Read more (PubMed: 22736761) »

    See all 2 Publications for this product

    Product Wall

    Application Immunoprecipitation
    Immuno-precipitation step Protein A/G
    Sample Mouse Cell lysate - whole cell (Neuro2A cell line)
    Specification Neuro2A cell line
    Treatment Transfected with NeuroD2-myc or emtpty vector pcDNA4
    Total protein in input 200 µg
    Username

    Abcam user community

    Verified customer

    提交于 Jul 11 2014

    Application Western blot
    Loading amount 10 µg
    Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
    Sample Mouse Cell lysate - whole cell (mouse N2A cell line)
    Specification mouse N2A cell line
    Treatment Transfected with NeuroD2 expressing vector
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    提交于 May 13 2014

    Application ChIP
    Detection step Other
    Sample Mouse Tissue lysate - whole (Cerebral cortex tissue from postnatal day 0 mouse)
    Specification Cerebral cortex tissue from postnatal day 0 mouse
    Negative control ChIP performed with unrelated antibody (anti-GFP antibody).
    Type Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% formaldehyde
    Positive control Known target of NeuroD2, which is the promoter regions of Nhlh2 gene. Primer sequence for PCR was: Nhlh2_F CTCACGAACTTCACCCGCAC Nhlh2_R AAATTGACTCCTCGGCCCTC
    Username

    Abcam user community

    Verified customer

    提交于 Apr 18 2014

    According to our records, ab109406 was proving difficult to use and we were in contact in order to help resolve the issue.

    Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand the ...

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    Thank you for contacting us and for letting us know about the trouble with ab109406.

    I have attached the protocol that was used by the lab to test this antibody.

    Could you tell me a bit more about the problem and the protocol used, ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"