概述

  • 产品名称Anti-NeuroD2抗体
    参阅全部 NeuroD2 一抗
  • 描述
    兔多克隆抗体to NeuroD2
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Chicken, Cow, Cat, Dog
  • 免疫原

    A region within synthetic peptide: VKEEGELGGE EEEEEEEEEG LDEAEGERPK KRGPKKRKMT KARLERSKLR, corresponding to N terminal amino acids 73-122 of Human NeuroD2

  • 阳性对照
    • Transfected 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab66607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 41 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:62500.

靶标

Anti-NeuroD2 antibody 图像

  • Anti-NeuroD2 antibody (ab66607) at 0.5 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 41 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)

Anti-NeuroD2 antibody (ab66607)参考文献

ab66607 has not yet been referenced specifically in any publications.

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