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A region within synthetic peptide: VKEEGELGGE EEEEEEEEEG LDEAEGERPK KRGPKKRKMT KARLERSKLR, corresponding to N terminal amino acids 73-122 of Human NeuroD2
- Transfected 293T cell lysate
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 0.5 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 41 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
Titre using peptide based assay: 1:62500.
Anti-NeuroD2 antibody 图像
Western blot - NeuroD2 antibody (ab66607)
Anti-NeuroD2 antibody (ab66607) at 0.5 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size :
41 kDaObserved band size :
48 kDa (why is the actual band size different from the predicted?
Anti-NeuroD2 antibody (ab66607)参考文献
has not yet been referenced specifically in any publications.
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