纯化说明The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
功能Differentiation factor required for dendrite morphogenesis and maintenance in the cerebellar cortex. Transcriptional activator. Binds to the insulin gene E-box.
疾病相关Defects in NEUROD1 are the cause of maturity-onset diabetes of the young type 6 (MODY6) [MIM:606394]. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
翻译后修饰Phosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335; which promotes dendritic growth.
neurogenic helix loop helix protein NEUROD antibody
Neuronal differentiation 1 antibody
Anti-NeuroD1 (phospho S274) antibody 图像
Western blot - Anti-NeuroD1 (phospho S274) antibody (ab78900)This image is courtesy of an Abreview submitted by Estela Munoz
All lanes : Anti-NeuroD1 (phospho S274) antibody (ab78900) at 1/5000 dilution
Lane 1 : Nuclear and cytoplasmic fractions from adult rat cerebellum Lane 2 : Nuclear and cytoplasmic fractions from adult rat cerebellum Lane 3 : Nuclear and cytoplasmic fractions from adult rat pancreas Lane 4 : Nuclear and cytoplasmic fractions from adult rat pancreas Lane 5 : Nuclear fractions from adult rat pineal gland pools at ZT14 Lane 6 : Nuclear fractions from adult rat pineal gland pools at ZT14 Lane 7 : Cytoplasmic fractions from adult pineal gland pools at ZT14 Lane 8 : Cytoplasmic fractions from adult pineal gland pools at ZT14
Lysates/proteins at 40 µg per lane.
Secondary Biotin-conjugated goat anti-rabbit IgG polclonal at 1/50000 dilution Developed using the ECL technique
Castro AE et al. Expression and cellular localization of the transcription factor NeuroD1 in the developing and adult rat pineal gland. J Pineal Res58:439-51 (2015).
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