Anti-NeuroD1 (phospho S274)抗体(ab78900)

概述

  • 产品名称Anti-NeuroD1 (phospho S274)抗体
    参阅全部 NeuroD1 一抗
  • 描述
    兔多克隆抗体to NeuroD1 (phospho S274)
  • 特异性ab78900 detects endogenous levels of NeuroD1 only when phosphorylated at serine 274.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    Synthesized phosphopeptide derived from human NeuroD1 around the phosphorylation site of serine 274 (P-L-SP-P-P).

  • 阳性对照
    • Extracts from HeLa cells, treated with UV (15 min)

性能

应用

Our Abpromise guarantee covers the use of ab78900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 40 kDa.
ELISA 1/10000.

靶标

  • 功能Differentiation factor required for dendrite morphogenesis and maintenance in the cerebellar cortex. Transcriptional activator. Binds to the insulin gene E-box.
  • 疾病相关Defects in NEUROD1 are the cause of maturity-onset diabetes of the young type 6 (MODY6) [MIM:606394]. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • 序列相似性Contains 1 basic helix-loop-helix (bHLH) domain.
  • 翻译后修饰Phosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335; which promotes dendritic growth.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • atonal antibody
    • basic helix loop helix transcription factor antibody
    • BETA 2 antibody
    • Beta cell E box transactivator 2 antibody
    • BETA2 antibody
    • BHF 1 antibody
    • BHF1 antibody
    • bHLHa3 antibody
    • class A basic helix loop helix protein 3 antibody
    • Class A basic helix-loop-helix protein 3 antibody
    • MODY 6 antibody
    • MODY6 antibody
    • NDF1_HUMAN antibody
    • NeuroD antibody
    • NeuroD1 antibody
    • Neurogenic differentiation 1 antibody
    • Neurogenic differentiation factor 1 antibody
    • neurogenic helix loop helix protein NEUROD antibody
    • Neuronal differentiation 1 antibody
    see all

Anti-NeuroD1 (phospho S274) antibody 图像

  • All lanes : Anti-NeuroD1 (phospho S274) antibody (ab78900) at 1/5000 dilution

    Lane 1 : Nuclear and cytoplasmic fractions from adult rat cerebellum
    Lane 2 : Nuclear and cytoplasmic fractions from adult rat cerebellum
    Lane 3 : Nuclear and cytoplasmic fractions from adult rat pancreas
    Lane 4 : Nuclear and cytoplasmic fractions from adult rat pancreas
    Lane 5 : Nuclear fractions from adult rat pineal gland pools at ZT14
    Lane 6 : Nuclear fractions from adult rat pineal gland pools at ZT14
    Lane 7 : Cytoplasmic fractions from adult pineal gland pools at ZT14
    Lane 8 : Cytoplasmic fractions from adult pineal gland pools at ZT14

    Lysates/proteins at 40 µg per lane.

    Secondary
    Biotin-conjugated goat anti-rabbit IgG polclonal at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes

    This image is courtesy of an Abreview submitted by Estela Munoz

    See Abreview

  • All lanes : Anti-NeuroD1 (phospho S274) antibody (ab78900) at 1/500 dilution

    Lane 1 : extracts from HeLa cells, treated with UV (15 min) with no immunizing peptide
    Lane 2 : extracts from HeLa cells, treated with UV (15 min) with immunizing peptide at 5 µg

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 40 kDa
    Observed band size : 40 kDa

Anti-NeuroD1 (phospho S274) antibody (ab78900)参考文献

This product has been referenced in:
  • Castro AE  et al. Expression and cellular localization of the transcription factor NeuroD1 in the developing and adult rat pineal gland. J Pineal Res 58:439-51 (2015). WB ; Rat . Read more (PubMed: 25752781) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Rat Tissue lysate - other (Pineal Gland)
Gel Running Conditions Reduced Denaturing
Loading amount 40 µg
Specification Pineal Gland
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Estela Munoz

Verified customer

提交于 Jun 24 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"