Anti-Neurocan抗体[650.24] (ab31979)

概述

  • 产品名称Anti-Neurocan抗体[650.24]
    参阅全部 Neurocan 一抗
  • 描述
    小鼠单克隆抗体[650.24] to Neurocan
  • 特异性Specific for Neurocan core protein; also reacts with neurocan C fragment
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Embryonic rat brain proteoglycans

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号650.24
  • 骨髓瘤P3x63
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab31979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 143 kDa.

靶标

  • 功能May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid.
  • 组织特异性Brain.
  • 序列相似性Belongs to the aggrecan/versican proteoglycan family.
    Contains 1 C-type lectin domain.
    Contains 2 EGF-like domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    Contains 2 Link domains.
    Contains 1 Sushi (CCP/SCR) domain.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Chondroitin sulfate proteoglycan 3 antibody
    • Cspg3 antibody
    • Ncan antibody
    • NCAN_HUMAN antibody
    • NEUR antibody
    • Neurocan core protein antibody
    • Neurocan proteoglycan antibody
    see all

Anti-Neurocan antibody [650.24] 图像

  • ab31979 staining Neurocan in rat glioma cell line C6 by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab31979 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated goat anti-mouse polyclonal, used at a 1/300 dilution. Nuclei are counterstained with DAPI.

    See Abreview

Anti-Neurocan antibody [650.24] (ab31979)参考文献

ab31979 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

According to the Unigen expression profile of this protein, a good positive control for this protein could be brain or vascular mouse tissue lysates.

http://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer....

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human glioblastoma cell line)
Specification human glioblastoma cell line
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jan 23 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (glioblastoma cell line C6)
Specification glioblastoma cell line C6
Fixative Paraformaldehyde
Permeabilization Yes - 0,1% Triton X 100 in PBS
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jan 23 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (glioma cell line C6)
Specification glioma cell line C6
Fixative Paraformaldehyde
Permeabilization Yes - 0,1% Triton X 100
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5%
Username

Abcam user community

Verified customer

提交于 Aug 31 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human glioblastoma cell line D54MG)
Specification human glioblastoma cell line D54MG
Fixative Paraformaldehyde
Permeabilization Yes - 0,1% Triton X 100
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Aug 31 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"