Anti-Neurobeachin抗体(ab58563)

概述

  • 产品名称
    Anti-Neurobeachin抗体
    参阅全部 Neurobeachin 一抗
  • 描述
    山羊多克隆抗体to Neurobeachin
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Dog, Pufferfish, Zebrafish
  • 免疫原

    Synthetic peptide:

    C-DFNRWHYEHQNRY

    , corresponding to C terminal amino acids 2934-2946 of Human Neurobeachin

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab58563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明
    This antibody gave a positive result in Peptide ELISA against the immunizing peptide. Antibody detection limit dilution 1/8000.


    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 300 kDa (predicted molecular weight: 327 kDa). Approx 300 kDa band observed in lysates of transfected HEK293 and endogenously in lysates of insulinoma cell line B-TC3.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Binds to type II regulatory subunits of protein kinase A and anchors/targets them to the membrane. May anchor the kinase to cytoskeletal and/or organelle-associated proteins.
    • 组织特异性
      Predominant in many brain structures. Also expressed at medium levels in spleen, thymus, prostate, testis and ovary. Low level expression is seen in heart, kidney, pancreas, skeletal muscle and intestine.
    • 序列相似性
      Belongs to the WD repeat neurobeachin family.
      Contains 1 BEACH domain.
      Contains 5 WD repeats.
    • 结构域
      RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
    • 细胞定位
      Cytoplasm. Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BCL8B antibody
      • FLJ10197 antibody
      • KIAA1544 antibody
      • Lysosomal trafficking regulator 2 antibody
      • Lysosomal-trafficking regulator 2 antibody
      • LYST2 antibody
      • NBEA antibody
      • NBEA_HUMAN antibody
      • Neurobeachin antibody
      • Protein BCL8B antibody
      • Protein neurobeachin antibody
      • RP11-270C18.1 antibody
      see all

    Anti-Neurobeachin antibody 图像

    • All lanes : Anti-Neurobeachin antibody (ab58563) at 1 µg/ml

      Lane 1 : Lysate prepared from non transfected HEK293 T cells
      Lane 2 : Lysate prepared from HEK293 T cells transfected with mouse Neurobeachin

      Developed using the ECL technique

      Predicted band size : 327 kDa
      Observed band size : 300 kDa (why is the actual band size different from the predicted?)

    实验方案

    Anti-Neurobeachin antibody (ab58563)参考文献

    ab58563 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab58563.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册