Anti-NRXN2抗体(ab34245)
Key features and details
- Rabbit polyclonal to NRXN2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-NRXN2抗体 -
描述
兔多克隆抗体to NRXN2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Rat, Human
预测可用于: Mouse -
免疫原
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阳性对照
- normal adult human cerebral cortex SKNSH cell line
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab34245于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 5 µg/ml.
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说明 |
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WB
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 5 µg/ml. |
靶标
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相关性
Neurexin 2 alpha belongs to the Neurexin family, a group of neuronal proteins that function as cell adhesion molecules during synaptogenesis and intracellular signaling. Mammalian genomes contain just three neurexin genes. However, many (>200) neurexin isoforms may be expressed through the use of two alternative promoters and alternative splicing at up to five different positions. -
细胞定位
Membrane; single-pass type I membrane protein. -
数据库链接
- Entrez Gene: 9379 Human
- Entrez Gene: 18190 Mouse
- Entrez Gene: 116595 Rat
- SwissProt: Q9P2S2 Human
- SwissProt: Q63374 Rat
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别名
- Neurexin 2 antibody
- Neurexin II antibody
- NRXN2 antibody
图片
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ICC/IF image of ab34245 stained SKNSH cells. The cells were 4% formaldehdye fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34245, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab34245 被引用在 4 文献中.
- Harkin LF et al. Neurexins 1-3 Each Have a Distinct Pattern of Expression in the Early Developing Human Cerebral Cortex. Cereb Cortex 27:216-232 (2017). PubMed: 28013231
- Brito-Moreira J et al. Interaction of amyloid-ß (Aß) oligomers with neurexin 2a and neuroligin 1 mediates synapse damage and memory loss in mice. J Biol Chem 292:7327-7337 (2017). PubMed: 28283575
- Shapiro-Reznik M et al. Diurnal rhythms in neurexins transcripts and inhibitory/excitatory synapse scaffold proteins in the biological clock. PLoS One 7:e37894 (2012). WB ; Rat . PubMed: 22662246
- Borsics T et al. Subcellular distribution and expression of prenylated Rab acceptor 1 domain family, member 2 (PRAF2) in malignant glioma: Influence on cell survival and migration. Cancer Sci : (2010). IHC-P ; Human . PubMed: 20412121