Anti-Neuraminidase抗体(ab98178)

概述

  • 产品名称
    Anti-Neuraminidase抗体
    参阅全部 Neuraminidase 一抗
  • 描述
    兔多克隆抗体to Neuraminidase
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Dog, Pig, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human Neuraminidase.

    (Peptide available as ab110204.)

  • 阳性对照
    • This antibody gave a positive signal in Human heart tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab98178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

靶标

  • 功能
    Catalyzes the removal of sialic acid (N-acetylneuramic acid) moities from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage.
  • 组织特异性
    Highly expressed in pancreas, followed by skeletal muscle, kidney, placenta, heart, lung and liver. Weakly expressed in brain.
  • 疾病相关
    Defects in NEU1 are the cause of sialidosis (SIALIDOSIS) [MIM:256550]. It is a lysosomal storage disease occurring as two types with various manifestations. Type 1 sialidosis (cherry red spot-myoclonus syndrome or normosomatic type) is late-onset and it is characterized by the formation of cherry red macular spots in childhood, progressive debilitating myoclonus, insiduous visual loss and rarely ataxia. The diagnosis can be confirmed by the screening of the urine for sialyloligosaccharides. Type 2 sialidosis (also known as dysmorphic type) occurs as several variants of increasing severity with earlier age of onset. It is characterized by the presence of abnormal somatic features including coarse facies and dysostosis multiplex, vertebral deformities, mental retardation, cherry-red spot/myoclonus, sialuria, cytoplasmic vacuolation of peripheral lymphocytes, bone marrow cells and conjunctival epithelial cells.
  • 序列相似性
    Belongs to the glycosyl hydrolase 33 family.
    Contains 4 BNR repeats.
  • 结构域
    A C-terminal internalization signal (YGTL) appears to allow the targeting of plasma membrane proteins to endosomes.
  • 翻译后修饰
    N-glycosylated.
    Phosphorylation of tyrosine within the internalization signal results in inhibition of sialidase internalization and blockage on the plasma membrane.
  • 细胞定位
    Lysosome membrane. Lysosome lumen. Cell membrane. Cytoplasmic vesicle. Localized not only on the inner side of the lysosomal membrane and in the lysosomal lumen, but also on the plasma membrane and in intracellular vesicles.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Acetylneuraminyl hydrolase antibody
    • exo-alpha-sialidase antibody
    • G9 sialidase antibody
    • Lysosomal sialidase antibody
    • N acetyl alpha neuraminidase 1 antibody
    • N-acetyl-alpha-neuraminidase 1 antibody
    • NANH antibody
    • NEU antibody
    • NEU1 antibody
    • NEUR1_HUMAN antibody
    • Neuraminidase 1 antibody
    • Neuraminidase antibody
    • SIAL1 antibody
    • sialidase 1 (lysosomal sialidase) antibody
    • Sialidase 1 antibody
    • Sialidase, lysosomal antibody
    • Sialidase-1 antibody
    see all

图片

  • Anti-Neuraminidase antibody (ab98178) at 1/250 dilution + Human heart tissue lysate - total protein (ab29431) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 45 kDa


    Exposure time : 8 minutes

实验方案

文献

ab98178 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
ELISA
Sample
Human Recombinant protein (HEK293 cell line)
Specification
HEK293 cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 27°C
Type
Indirect
Username

Dr. Shivakumar Devaiah

Verified customer

提交于 Feb 23 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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