Anti-Netrin G2 Ligand抗体(ab116697)


  • 产品名称
    Anti-Netrin G2 Ligand抗体
    参阅全部 Netrin G2 Ligand 一抗
  • 描述
    兔多克隆抗体to Netrin G2 Ligand
  • 宿主
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Horse, Dog, Human, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 550 to the C-terminus of Mouse Netrin G2 Ligand.

  • 阳性对照
    • This antibody gave a positive signal in the following Mouse tissue lysates: Brain; Hippocampus; Forebrain; Cortex.


  • 形式
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
  • 同种型
  • 研究领域


Our Abpromise guarantee covers the use of ab116697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 73 kDa).


  • 功能
    Synaptic adhesion protein. Regulates the formation of exitatory synapses through the recruitement of pre-and-postsynaptic proteins. Organize the lamina/pathway-specific differentiation of dendrites. Plays a important role for auditory synaptic responses. Involved in the suppression of glioma.
  • 组织特异性
    Specifically expressed in brain.
  • 序列相似性
    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 9 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
  • 结构域
    The last 4 C-terminal residues bind to the first 2 PDZ domains of DLG4.
  • 翻译后修饰
  • 细胞定位
    Membrane. Cell junction > synapse > postsynaptic cell membrane. LRRC4 and DLG4 are interdependent for synaptic localization.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BAG antibody
    • Brain tumor associated protein LRRC4 antibody
    • Brain tumor-associated protein BAG antibody
    • Leucine rich repeat containing 4 antibody
    • Leucine rich repeat containing protein 4 antibody
    • Leucine-rich repeat-containing protein 4 antibody
    • Lrrc4 antibody
    • LRRC4_HUMAN antibody
    • MGC133342 antibody
    • MGC133343 antibody
    • NAG14 antibody
    • Nasopharyngeal carcinoma associated gene 14 protein antibody
    • Nasopharyngeal carcinoma-associated gene 14 protein antibody
    • Netrin G2 ligand antibody
    • Netrin-G2 ligand antibody
    • NGL 2 antibody
    • NGL-2 antibody
    • OTTHUMP00000212282 antibody
    • OTTHUMP00000212283 antibody
    • OTTHUMP00000212284 antibody
    see all


  • All lanes : Anti-Netrin G2 Ligand antibody (ab116697) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Mouse Hippocampus Tissue Lysate
    Lane 3 : Forebrain (Mouse) Tissue Lysate
    Lane 4 : Mouse Cortex Tissue Lysate
    Lane 5 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate - Negative control

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 73 kDa
    Observed band size: 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 37 kDa, 44 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 12 minutes

    Netrin G2 Ligand contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Please note that U87MG was used as a negative control. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab116697 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.



ab116697 has not yet been referenced specifically in any publications.


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