产品名称 Anti-NET1抗体 参阅全部 NET1 一抗
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Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae
Synthetic peptide, corresponding to a region within internal sequence amino acids 287-336 (AILIIQGVLSDINLKKGESECQYYIDKLEYLDEKQRDPRIEASKVLLCH G) of Human NET1 (NP_005854).
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
存储溶液 Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
功能 Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.
组织特异性 Widely expressed.
序列相似性 Contains 1 DH (DBL-homology) domain. Contains 1 PH domain.
细胞定位 Cytoplasm. Nucleus.
Information by UniProt
Anti-NET1 antibody 图像
Western blot - NET1 antibody (ab84615)
Anti-NET1 antibody (ab84615) at 1 µg/ml + Jurkat cell lysate at 10 µg Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution Predicted band size : 62 kDa Observed band size : 65 kDa ( why is the actual band size different from the predicted?) Additional bands at : >90 kDa. We are unsure as to the identity of these extra bands.
Anti-NET1 antibody (ab84615)参考文献
has not yet been referenced specifically in any publications.
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