概述

  • 产品名称Anti-NET1抗体
    参阅全部 NET1 一抗
  • 描述
    山羊多克隆抗体to NET1
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide: DKALSGGKRKETLV, corresponding to C terminal amino acids 529-542 of Human NET1.

  • 常规说明GenBank Accession Number – NP_005854

性能

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应用

Our Abpromise guarantee covers the use of ab5914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Can be blocked with Human NET1 peptide (ab23120).
ELISA 1/32000.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.
  • 组织特异性Widely expressed.
  • 序列相似性Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARHG8_HUMAN antibody
    • ARHGEF8 antibody
    • GLYT2 antibody
    • Guanine nucleotide regulatory protein oncogene antibody
    • MGC114559 antibody
    • MGC127767 antibody
    • MGC53602 antibody
    • mNET1 antibody
    • Net1 antibody
    • NET1A antibody
    • Neuroepithelial cell transforming gene 1 antibody
    • Neuroepithelial cell-transforming gene 1 protein antibody
    • p65 Net1 proto oncogene antibody
    • Proto-oncogene p65 Net1 antibody
    • Rho guanine nucleotide exchange factor 8 antibody
    • Rho guanine nucleotide exchange factor GEF 8 antibody
    see all

Anti-NET1 antibody 图像

  • ab5914 (2.5 µg/ml) staining NET1 in Human Tonsil by IHC-P.  Steamed antigen retrieval with citrate buffer pH 6, AP-staining.  This image shows staining of fibrous structures.

Anti-NET1 antibody (ab5914)参考文献

This product has been referenced in:
  • Levi L  et al. Bacterial genotoxins promote inside-out integrin ß1 activation, formation of focal adhesion complexes and cell spreading. PLoS One 10:e0124119 (2015). WB . Read more (PubMed: 25874996) »
  • Srougi MC & Burridge K The Nuclear Guanine Nucleotide Exchange Factors Ect2 and Net1 Regulate RhoB-Mediated Cell Death after DNA Damage. PLoS One 6:e17108 (2011). WB ; Human . Read more (PubMed: 21373644) »

See all 4 Publications for this product

Product Wall

We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody ...

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