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Our Abpromise guarantee covers the use of ab5914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Can be blocked with Human NET1 peptide (ab23120).|
|IHC-P||Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab5914 (2.5 µg/ml) staining NET1 in Human Tonsil by IHC-P. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This image shows staining of fibrous structures.