RabMAb

Anti-Nestin抗体[SP103] (ab105389)

概述

  • 产品名称Anti-Nestin抗体[SP103]
    参阅全部 Nestin 一抗
  • 描述
    兔单克隆抗体[SP103] to Nestin
  • 经测试应用适用于: ICC/IF, Flow Cyt, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to the C terminus of Human Nestin.

  • 阳性对照
    • HeLa cell lysate and Human kidney tissue. SKNSH cell line

性能

应用

Our Abpromise guarantee covers the use of ab105389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/100. Predicted molecular weight: 177 kDa.
IHC-P 1/100.

Antigen retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.

靶标

  • 功能Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
  • 组织特异性CNS stem cells.
  • 序列相似性Belongs to the intermediate filament family.
  • 发展阶段Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
  • 翻译后修饰Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ESTM 46 antibody
    • FLJ 21841 antibody
    • FLJ21841 antibody
    • Intermediate filament protein antibody
    • Nbla00170 antibody
    • nes antibody
    • NEST_HUMAN antibody
    • Nestin antibody
    see all

Anti-Nestin antibody [SP103] 图像

  • Anti-Nestin antibody [SP103] (ab105389) at 1/100 dilution + HeLa cell lysate

    Predicted band size : 177 kDa
  • Immunohistochemical staining of human kidney with ab105389 at 1/100.

  • ab105389 staining Nestin in human melanoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using Tris EDTA pH 9.0. Samples were then permeabilized using 0.25% Triton X-100, blocked, then incubated with ab105389 at a 1/100 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 594 (red) conjugated donkey anti-rabbit polyclonal used at a 1/100 dilution. (Green) tumor cells, (blue) DAPI.

    See Abreview

  • ICC/IF image of ab105389 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105389, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Overlay histogram showing SH-SY5Y cells stained with ab105389 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab105389, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Nestin antibody [SP103] (ab105389)参考文献

This product has been referenced in:
  • Zhao W  et al. Effect of TGF-ß1 on the Migration and Recruitment of Mesenchymal Stem Cells after Vascular Balloon Injury: Involvement of Matrix Metalloproteinase-14. Sci Rep 6:21176 (2016). IHC-P ; Rat . Read more (PubMed: 26880204) »
  • Tam J  et al. Reconstitution of full-thickness skin by microcolumn grafting. J Tissue Eng Regen Med N/A:N/A (2016). IHC-P ; Human . Read more (PubMed: 27296503) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Dako protein block as blocking agent for 10 minute(s) · Concentration: .25% · Temperature: 22°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Sample Dog Tissue sections (Fetal tissue)
Specification Fetal tissue
Permeabilization No
Fixative Formaldehyde
Username

Ms. Erica Sloma

Verified customer

提交于 Aug 21 2014

Application Western blot
Loading amount 5 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - other (undifferentiated human iPS cells)
Specification undifferentiated human iPS cells
Blocking step 5% milk in TBST as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Dec 18 2013

Application Immunocytochemistry/ Immunofluorescence
Blocking step 1% serum, 0.1% triton, 0.1% BSA in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Sample Human Cell (neural progenitor cell derived from human iPSCs)
Specification neural progenitor cell derived from human iPSCs
Permeabilization Yes - (see blocking buffer)
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 22 2013

Thank you for contacting us. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. Read More

Thank you for contacting us.

Unfortunately, alignments of the immunogen with mouse show very poor homology. As such, we cannot guarantee or recommend this product for use in this species. We do offer a number of other anti-Nestin antibodie...

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The concentration of lot in question is XXXug/ml (or XX mg/ml).

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (kidney)
Specification kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA pH9, 20 min, 100C
Permeabilization No
Username

Abcam user community

Verified customer

提交于 Nov 22 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Melanoma)
Specification Melanoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris EDTA pH9.0
Permeabilization Yes - 0.25% Triton X-100
Blocking step DakoCytomation X0909 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Hongwei Shao

Verified customer

提交于 Nov 09 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"