Anti-Nestin抗体- Neural Stem Cell Marker (ab92391)

概述

  • 产品名称Anti-Nestin抗体- Neural Stem Cell Marker
    参阅全部 Nestin 一抗
  • 描述
    兔多克隆抗体to Nestin - Neural Stem Cell Marker
  • 特异性The immunoreactivity detected with Rabbit polyclonal to Nestin (ab92391) does not overlap in with that of the mature neuronal markers such as MAP2, NeuN, Neurofilament L and Beta-3-tublin (data available on request).
  • 经测试应用适用于: ICC/IFmore details
    不适用于: IHC-FoFr or IHC-P
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1600 to the C-terminus of Human Nestin.

    (Peptide available as ab19375.)

  • 阳性对照
    • This antibody gave a positive signal in P0 rat retinal cell culture and E16 rat hippocampal cell culture.
  • 常规说明ab92391 is raised using the same immunogen as ab5968. Batches sold under ab92391 have passed QC testing in ICC/IF only. In our hands, this product did not work in IHC-P. For further information please contact our Customer Services team.

性能

应用

Our Abpromise guarantee covers the use of ab92391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/250.
  • 应用说明Is unsuitable for IHC-FoFr or IHC-P.
  • 靶标

    • 功能Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
    • 组织特异性CNS stem cells.
    • 序列相似性Belongs to the intermediate filament family.
    • 发展阶段Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
    • 翻译后修饰Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ESTM 46 antibody
      • FLJ 21841 antibody
      • FLJ21841 antibody
      • Intermediate filament protein antibody
      • Nbla00170 antibody
      • nes antibody
      • NEST_HUMAN antibody
      • Nestin antibody
      see all

    Anti-Nestin antibody - Neural Stem Cell Marker 图像

    • Immunocytochemical localisation of Nestin using ab92391 on P0 rat retinal culture. The image shows a nestin positive retinal ganglionic cell from P0 retina that has been grown for 7 days on a glass coverslip. Cover slips were fixed in 5% PFA in 0.1M PBS (1xPBS) for 5 minutes and washed with 0.1M PBS. Permeabilisation: 0.3% Triton X-100. Primary antibody ab92391 was diluted 1/250 and incubated for 2h @ RT. Secondary antibody: donkey anti-rabbit Alexa Fluor 488 (1/2000).
    • Immunocytochemical localisation of Nestin using ab92391 on E16 rat hippocampal cell culture. The image shows nestin positive rat hippocampal cells isolated from embryonic day 16 (grown in serum free conditions for 2-3 weeks). Cover slips were fixed in 5% PFA in 0.1M PBS (1xPBS) for 5 minutes and washed with 0.1M PBS. Permeabilisation: 0.3% Triton X-100. Primary antibody ab92391 was diluted 1/250 and incubated for 2h @ RT. Secondary antibody: donkey anti-rabbit Alexa Fluor 488 (1/2000). DAPI in blue stains nuclei.
    • ICC/IF image of ab92391 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92391, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Anti-Nestin antibody - Neural Stem Cell Marker (ab92391)参考文献

    This product has been referenced in:
    • Fu S  et al. Aberrant adult Neurogenesis in the subventricular zone-rostral migratory stream-olfactory bulb system following subchronic manganese exposure. Toxicol Sci N/A:N/A (2016). Read more (PubMed: 26794142) »
    • Jaszberenyi M  et al. Potentiation of cytotoxic chemotherapy by growth hormone-releasing hormone agonists. Proc Natl Acad Sci U S A 111:781-6 (2014). WB . Read more (PubMed: 24379381) »

    See all 4 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (corneal endothelium)
    Specification corneal endothelium
    Fixative Paraformaldehyde
    Permeabilization Yes - 1% triton
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C
    Username

    Dr. Zhiguo He

    Verified customer

    提交于 Nov 01 2012

    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Rat Tissue sections (Brain)
    Specification Brain
    Fixative Paraformaldehyde
    Permeabilization No
    Username

    Dr. Sophie Pezet

    Verified customer

    提交于 Sep 21 2012

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Rat Tissue sections (Brain sections)
    Specification Brain sections
    Fixative Paraformaldehyde
    Antigen retrieval step None
    Username

    Dr. Sophie Pezet

    Verified customer

    提交于 May 26 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"